Protein-spatiotemporal partition releasing gradient porous scaffolds and anti-inflammatory and antioxidant regulation remodel tissue engineered anisotropic meniscus  

作　　者：Bingbing Xu Jing Ye Bao-Shi Fan Xinjie Wang Ji-Ying Zhang Shitang Song Yifan Song Wen-Bo Jiang Xing Wang Jia-Kuo Yu 

机构地区：[1]Sports Medicine Department,Beijing Key Laboratory of Sports Injuries,Peking University Third Hospital,No.49,North Garden Road,Haidian District,Beijing 100191,PR China [2]Peking University Institute of Sports Medicine,No.49,North Garden Road,Haidian District,Beijing 100191,PR China [3]Beijing National Laboratory for Molecular Sciences,State Key Laboratory of Polymer Physics&Chemistry,Institute of Chemistry,Chinese Academy of Sciences,Beijing,100190,PR China [4]University of Chinese Academy of Sciences,Beijing,100049,PR China [5]Clinical Translational R&D Center of 3D Printing Technology,Shanghai Ninth People’s Hospital,Shanghai Jiao Tong University School of Medicine,Shanghai,200011,PR China

出　　处：《Bioactive Materials》2023年第2期194-207,共14页生物活性材料（英文）

基　　金：the National Natural Science Foundation of China(NSFC,82002298,51920105006,51973226);the China Postdoctoral Science Foundation(2020M670066);the National Key Research and Development Program of China(2016YFC1100704);the Youth Innovation Promotion Association CAS(2019031).

摘　　要：Meniscus is a wedge-shaped fibrocartilaginous tissue,playing important roles in maintaining joint stability and function.Meniscus injuries are difficult to heal and frequently progress into structural breakdown,which then leads to osteoarthritis.Regeneration of heterogeneous tissue engineering meniscus(TEM)continues to be a scientific and translational challenge.The morphology,tissue architecture,mechanical strength,and functional applications of the cultivated TEMs have not been able to meet clinical needs,which may due to the negligent attention on the importance of microenvironment in vitro and in vivo.Herein,we combined the 3D(three-dimensional)-printed gradient porous scaffolds,spatiotemporal partition release of growth factors,and anti-inflammatory and anti-oxidant microenvironment regulation of Ac2-26 peptide to prepare a versatile meniscus composite scaffold with heterogeneous bionic structures,excellent biomechanical properties and anti-inflammatory and anti-oxidant effects.By observing the results of cell activity and differentiation,and biomechanics under anti-inflammatory and anti-oxidant microenvironments in vitro,we explored the effects of anti-inflammatory and anti-oxidant microenvironments on construction of regional and functional heterogeneous TEM via the growth process regulation,with a view to cultivating a high-quality of TEM from bench to bedside.

关 键 词：Tissue engineering meniscus Gradient porous scaffolds Spatiotemporal partition release Ac2-26 peptide Anti-inflammatory and anti-oxidant regulation 

分 类 号：R318.08[医药卫生—生物医学工程]