Modification of adipose mesenchymal stem cells-derived small extracellular vesicles with fibrin-targeting peptide CREKA for enhanced bone repair  

作　　者：Qi Wu Xiaoling Fu Xian Li Jing Li Weiju Han Yingjun Wang 

机构地区：[1]School of Biomedical Sciences and Engineering,South China University of Technology,Guangzhou International Campus,Guangzhou,511442,PR China [2]National Engineering Research Center for Tissue Restoration and Reconstruction,South China University of Technology,Guangzhou,510006,PR China [3]Key Laboratory of Biomedical Engineering of Guangdong Province,South China University of Technology,Guangzhou,510006,PR China [4]Key Laboratory of Biomedical Materials and Engineering of the Ministry of Education,South China University of Technology,Guangzhou,510006,PR China

出　　处：《Bioactive Materials》2023年第2期208-220,共13页生物活性材料（英文）

基　　金：supported by the National Key R&D Program of China(2021YFB3800900);National Natural Science Foundation of China(31971266,51232002);the Key Research and Development Program of Guangzhou(202007020002);Guangdong Province Basic and Applied Research Foundation(2022A1515011925).

摘　　要：The process of bone repair is highly regulated by a large number of bioactive factors.Thus,a“cocktail”of bioactive factors supplemented to the defect sites is desirable for bone repair.In this regard,small extracellular vesicles(sEVs)derived from mesenchymal stem cells hold great potential in tissue repair.Nevertheless,the poor homing and retention of sEVs greatly limited their possible clinical application.In the present work,DMPE-PEG-CREKA was inserted into the membrane of sEVs released from adipose-derived mesenchymal stem cells to obtain CREKA functionalized sEVs(CREKA-sEVs),which could target fibrin to accumulate and retain in bone defects.Our results showed that CREKA-sEVs,like sEVs,promoted the osteogenic differentiation of BMSCs,the angiogenic property of HUVECs,and modulated the polarization of macrophages in vitro.Furthermore,due to the improved fibrin-binding and retention capacity of CREKA-sEVs,they enhanced the bone repair substantially in the rat femoral defect model.This study provided a new strategy to improve the therapeutic efficiency of sEVs and showed that CREKA-sEVs had great application value in bone tissue repair.

关 键 词：MSCs-derived sEVs Fibrin-targeting CREKA Bone repair 

分 类 号：R68[医药卫生—骨科学]