Unveiling the nanotoxicological aspects of Se nanomaterials differing in size and morphology  

作　　者：Hana Stepankova Hana Michalkova Zbynek Splichal Lukas Richtera Pavel Svec Tomas Vaculovic Jan Pribyl Martin Kormunda Simona Rex Vojtech Adam Zbynek Heger 

机构地区：[1]Department of Chemistry and Biochemistry,Mendel University in Brno,Zemedelska 1,Brno,CZ-61300,Czech Republic [2]Central European Institute of Technology,Brno University of Technology,Purkynova 123,Brno,CZ-61200,Czech Republic [3]Department of Chemistry,Masaryk University,Kamenice 5,Brno,CZ-62500,Czech Republic [4]Institute of Laboratory Research on Geomaterials,Faculty of Natural Sciences,Comenius University in Bratislava,Mlynska dolina,Ilkovicova 6,84215 Bratislava,Slovak Republic [5]Central European Institute of Technology,Masaryk University,Kamenice 5,Brno,CZ-62500,Czech Republic [6]Department of Physics,Faculty of Science,J.E.Purkyne University,Pasteurova 1,Usti nad Labem,CZ-40096,Czech Republic

出　　处：《Bioactive Materials》2023年第2期489-500,共12页生物活性材料（英文）

基　　金：support from ERDF"Multidisciplinary research to increase application potential of nanomaterials in agricultural practice"(No.CZ.02.1.01/0.0/0.0/16_025/0007314)is gratefully acknowledged;We also acknowledge CF Nanobiotechnology(project no.LM2018127)and Research Infrastructure NanoEnviCz(project no.LM2018124)both supported by MEYS CR for perfect assistance with physico-chemical characterization of Se nanomaterials;The research was also carried out under the project CEITEC 2020(LQ1601)with financial support from the MEYS CR under the National Sustainability Programme II.

摘　　要：Although the general concept of nanotechnology relies on exploitation of size-dependent properties of nanoscaled materials,the relation between the size/morphology of nanoparticles with their biological activity remains not well understood.Therefore,we aimed at investigating the biological activity of Se nanoparticles,one of the most promising candidates of nanomaterials for biomedicine,possessing the same crystal structure,but differing in morphology(nanorods vs.spherical particles)and aspect ratios(AR,11.5 vs.22.3 vs.1.0)in human cells and BALB/c mice.Herein,we report that in case of nanorod-shaped Se nanomaterials,AR is a critical factor describing their cytotoxicity and biocompatibility.However,spherical nanoparticles(AR 1.0)do not fit this statement and exhibit markedly higher cytotoxicity than lower-AR Se nanorods.Beside of cytotoxicity,we also show that morphology and size substantially affect the uptake and intracellular fate of Se nanomaterials.In line with in vitro data,in vivo i.v.administration of Se nanomaterials revealed the highest toxicity for higher-AR nanorods followed by spherical nanoparticles and lower-AR nanorods.Moreover,we revealed that Se nanomaterials are able to alter intracellular redox homeostasis,and affect the acidic intracellular vesicles and cytoskeletal architecture in a size-and morphology-dependent manner.Although the tested nanoparticles were produced from the similar sources,their behavior differs markedly,since each type is promising for several various application scenarios,and the presented testing protocol could serve as a concept standardizing the biological relevance of the size and morphology of the various types of nanomaterials and nanoparticles.

关 键 词：Aspect ratio BIOCOMPATIBILITY NANOTOXICOLOGY NANORODS 

分 类 号：TB383.1[一般工业技术—材料科学与工程]