基于金纳米颗粒修饰二氧化硅纳米花的生物传感器构建及应用  

The construction and application of biosensor based on gold nanoparticles loaded SiO_(2)-nanoflowers

作　　者：刘瑞琪 周栖桐 张悦 贺莹[1] 高静[1] 马丽[1] LIU Ruiqi;ZHOU Xitong;ZHANG Yue;HE Ying;GAO Jing;MA Li(School of Chemical Engineering and Technology,Hebei University of Technology,Tianjin 300401,China)

机构地区：[1]河北工业大学化工学院,天津300401

出　　处：《化工学报》2023年第3期1247-1259,共13页CIESC Journal

基　　金：河北省自然科学基金项目(B2020202036)。

摘　　要：利用褶皱状二氧化硅纳米花(SiO_(2)NFs),通过原位还原法制备分散的金纳米颗粒修饰的氨基二氧化硅复合材料(Au@NH_(2)-SiO_(2)NFs)。利用扫描电镜(SEM)、透射电镜(TEM)、X射线光电子能谱(XPS)、X射线衍射(XRD)、傅里叶红外光谱仪(FT-IR)和元素分析(elemental mapping)对Au@NH_(2)-SiO_(2)NFs纳米材料进行表征。基于Au@NH_(2)-SiO_(2)NFs构建AChE/Au@NH_(2)-SiO_(2)NFs/GCE生物传感器。选择马拉硫磷和毒死蜱两种有机磷农药为代表,考察生物传感器的检测性能,其中马拉硫磷的检测范围是(1.00×10^(-11))~(1.00×10^(-5))mol/L,检测限为2.92×10^(-12)mol/L;毒死蜱的检测范围是(1.00×10^(-13))~(1.00×10^(-7))mol/L,检测限为5.60×10^(-14)mol/L。AChE/Au@NH_(2)-SiO_(2)NFs/GCE电极在实际样品检测中的回收率在90.7%~107.0%之间,并展现出良好的抗干扰性、储藏稳定性和重现性。Dispersed gold nanoparticles-modified amino-silica composites(Au@NH_(2)-SiO_(2))were prepared by in situ reduction using wrinkled silica nanoflowers(SiO_(2)NFs).Au@NH_(2)-SiO_(2)NFs nanomaterials were characterized by scanning electron microscopy(SEM),transmission electron microscopy(TEM),X-ray photoelectron spectroscopy(XPS),X-ray diffraction(XRD),Fourier-infrared spectroscopy(FT-IR)and elemental mapping.The AChE/Au@NH_(2)-SiO_(2)NFs/GCE biosensor was constructed based on Au@NH_(2)-SiO_(2)NFs composite material.The detection performance of biosensor was investigated by two organophosphorus pesticides,malathion and chlorpyrifos.Separately,the prepared biosensor exhibited wide linear ranges of(1.00×10^(-11))—(1.00×10-5)mol/L with the LOD of 2.92×10^(-12)mol/L for malathion and(1.00×10^(-13))—(1.00×10^(-7))mol/L with a LOD of 5.60×10^(-14)mol/L for chlorpyrifos.The AChE/Au@NH_(2)-SiO_(2)NFs/GCE biosensor was also used to detect real samples getting the recovery rate of 90.7%—107.0%.The AChE/Au@NH_(2)-SiO_(2)NFs/GCE biosensor has outstanding stability and sensitivity.

关 键 词：乙酰胆碱酯酶 复合材料 生物传感器 电化学 有机磷农药 

分 类 号：O657.14[理学—分析化学] TP212.3[理学—化学]