HPLC法测定菊明降压丸中五种成分的含量  

The content determination of five components in Juming Jiangya Wan by HPLC

作　　者：乔巧玉 刘峻男 邱昭昭 赵旻[1] 王淼[2] 赵春杰[1] QIAO Qiaoyu;LIU Junnan;QIU Zhaozhao;ZHAO Min;WANG Miao;ZHAO Chunjie(School of Pharmacy,Shenyang Pharmaceutical University,Shenyang 110016,China;School of Life Sciences and Biopharmacy,Shenyang 110016,China)

机构地区：[1]沈阳药科大学药学院,辽宁沈阳110016 [2]沈阳药科大学生命科学与生物制药学院,辽宁沈阳110016

出　　处：《沈阳药科大学学报》2023年第3期293-299,共7页Journal of Shenyang Pharmaceutical University

基　　金：辽宁省教育厅项目(2017LQN19)。

摘　　要：目的建立HPLC法测定菊明降压丸中五种成分含量的方法。方法采用Kromat Universil XB-C_(18)色谱柱(150 mm×2.1 mm,3μm),以乙腈-体积分数为0.1%磷酸水为流动相,在流速为0.2 mL·min^(-1),柱温为30℃,检测波长为284 nm和337 nm的条件下进行梯度洗脱,对其中的绿原酸、蒙花苷、木犀草素、芹菜素、橙黄决明素的含量进行测定。结果绿原酸、蒙花苷、木犀草素、芹菜素、橙黄决明素的质量浓度分别在20.9~334.1、3.7~59.1、7.0~111.9、0.7~11.9和2.1~33.5 mg·L^(-1)内与各自峰面积呈良好的线性关系,平均回收率分别为100.6%、99.9%、101.2%、98.6%和98.8%,RSD分别为1.5%、1.2%、1.1%、1.4%和1.0%(n=9)。结论重复性好,可为菊明降压丸质量控制提供科学的参考。Objective To establish a high performance liquid chromatography method for simultaneous determination of five components in Juming Jiangya Wan.Methods Gradient elution was performed using a Kromat Universil XB-C_(18)column(150 mm×2.1 mm,3.0μm).The mobile phase was acetonitrile-0.1%phosphoric acid,the flow rate was 0.2 mL·min^(-1),the detection wavelengths were 284 nm and 337 nm,and the column temperature was set at 30℃.The contents of chlorogenic acid,buddleoside,luteolin,apigenin,aurantio-obtusin were measured.Results The mass concentrations of chlorogenic acid,buddleoside,luteolin,apigenin,aurantio-obtusin had a good linear relationship with their respective peak areas at 20.9-334.1,3.7-59.1,7.0-111.9,0.7-11.9 and 2.1-33.5 mg·L^(-1).The average recoveries were 100.6%,99.9%,101.2%,98.6%and 98.8%.The RSD were 1.5%,1.2%,1.1%,1.4%and 1.0%(n=9).Conclusion This method can provide scientific reference for the quality control of Juming Jiangya Wan.

关 键 词：菊明降压丸 高效液相色谱法 含量测定 

分 类 号：R917[医药卫生—药物分析学]