白三烯C4及2型固有淋巴细胞在支气管肺发育不良中的变化及意义  

Changes and significance of leukotriene C4 and type 2 innate lymphoid cell in bronchopulmonary dysplasia

作　　者：季玮 朱玥 尧惠慈 卢红艳[1] JI Wei;ZHU Yue;YAO Huici;LU Hongyan(Department of Pediatrics,Affiliated Hospital of Jiangsu University,Zhenjiang 212001,China)

机构地区：[1]江苏大学附属医院儿科,江苏镇江212001

出　　处：《基础医学与临床》2023年第5期711-716,共6页Basic and Clinical Medicine

基　　金：国家自然科学基金(82171702);江苏省自然科学基金(BK20201226)。

摘　　要：目的探讨白三烯C4(LTC4)、2型固有淋巴细胞(ILC2)及相关细胞因子(IL-4、IL-5及IL-13)在小鼠支气管肺发育不良(BPD)肺组织中的动态变化。方法将C57BL/6小鼠随机分为空气组及高氧组,高氧组暴露于85%的氧气中1、7及14 d,复制BPD模型。HE染色观察小鼠肺组织病理改变;流式细胞测量术检测ILC2及CysLT1R+ILC2百分比;ELISA检测LTC4、IL-4、IL-5及IL-13含量;Western blot检测白三烯C4合酶(LTC4S)蛋白表达;RT-qPCR检测LTC4S及半胱氨酸白三烯1型受体(CysLT1R)mRNA表达。结果与空气组相比,高氧组ILC2及CysLT1R+ILC2百分比增加(P<0.05),且CysLT1R mRNA表达增加(P<0.05);高氧组IL-4、IL-5及IL-13表达水平均增加且高于同时间点空气组(P<0.05);高氧组LTC4含量、LTC4S蛋白及mRNA表达均明显增加(P<0.05)。结论在BPD中,LTC4增加并可能通过CysLT1R诱导ILC2及相关细胞因子(IL-4、IL-5及IL-13)增加。Objective To investigate the dynamic changes of leukotriene C4(LTC4),type 2 innate lymphoid cell(ILC2)and related cytokines(IL-4,IL-5 and IL-13)in the lung tissue of mice with bronchopulmonary dysplasia(BPD).Methods C57BL/6 mice were randomly divided into air group and hyperoxia group.The hyperoxia group was exposed to 85%oxygen for 1,7 and 14 days to replicate the BPD model.HE staining microscopy was used to observe the pathological changes of the lung tissue of mice.The percentages of ILC2 and CysLT1R+ILC2 were detected by flow cytometry.The contents of LTC4,IL-4,IL-5 and IL-13 were detected by ELISA.Western blot was used to detect the expression of LTC4-C synthase(LTC4S).RT-qPCR was used to detect the mRNA expression of LTC4S and cysteine leukotriene type 1 receptor(CysLT1R).Results Compared with air group,the percentage of ILC2 and CysLT1R+ILC2 in hyperoxia group and the expression of CysLT1R mRNA all increased(P<0.05);The expression of IL-4,IL-5 and IL-13 in the hyperoxia group increased and were higher than those in the air group at the same time point(P<0.05);LTC4 content,LTC4S protein and mRNA expression were significantly increased in hyperoxia group(P<0.05).Conclusions In BPD,LTC4 increases and may induce the increase of ILC2 and related cytokines(IL-4,IL-5 and IL-13)through CysLT1R.

关 键 词：白三烯C4 2型固有淋巴细胞 2型炎性反应 支气管肺发育不良 

分 类 号：R722.19[医药卫生—儿科]