A型肉毒毒素含量的ELISA检测法的建立与应用  

Establishment and application of ELISA for the detection of botulinum toxin type A

作　　者：王琴琴 刘博 支德娟[1] 王东升 Wang Qinqin;Liu Bo;Zhi Dejuan;Wang Dongsheng(School of Pharmacy,Lanzhou University,Lanzhou 730000,China)

机构地区：[1]兰州大学药学院,甘肃兰州730000

出　　处：《兰州大学学报（医学版）》2023年第1期20-24,31,共6页Journal of Lanzhou University（Medical Sciences）

基　　金：甘肃省自然科学基金资助项目(20JR10RA619);国家级大学生创新创业训练计划项目(202110730220)。

摘　　要：目的 建立并应用A型肉毒毒素(BoNT/A)含量的ELISA检测方法。方法 采用免疫学技术,制备并纯化了兔源性抗BoNT/A切割突触小体相关蛋白SNAP-25后暴露出的八肽序列SNAP-25 (190-197)特异性抗体,建立BoNT/A含量的ELISA检测方法,且进行方法学验证,完成相关样品检测。结果 成功制备并纯化获得八肽兔多克隆抗体,建立的BoNT/A含量ELISA检测方法具有良好的灵敏度、特异性、重复性、适用性等优点。结论 成功建立了BoNT/A含量的ELISA检测方法,可用于相关样品的BoNT/A含量检测。Objective To establish and apply a detection method of ELISA for the content of botulinum toxin type A(BoNT/A).Methods An antibody against octapeptide(190-197)of synaptosomal-associated protein of 25 kDa(SNAP-25)produced by cleavage with BoNT/A was prepared and purified from rabbit serum.Then,ELISA for the content detection of BoNT/A was built,validated and used to measure the related samples.Results The antibody of octapeptide was successfully obtained and purified from rabbits,and the ELISA of the content detection method for BoNT/A,which was developed in this paper,was sensitive,specific,repeatable and applicable.Conclusion An ELISA of the content detection method for BoNT/A was successfully developed,and can be used to measure the content of BoNT/A in related samples.

关 键 词：A型肉毒毒素 八肽兔多抗 酶联免疫吸附分析 

分 类 号：R91[医药卫生—药学]