野生老芒麦种质资源鉴定与基部叶鞘绒毛变异分析  

Identification of Wild Elymus sibiricus Germplasm Resources and Analysis of Variation of Dorsal Hairs in Basal Leaf Sheath

作　　者：李明峰 李欣瑞 李英主 陈丽丽[2] 王艳[1] 李春艳 常丹[2] 雷雄[2] 闫利军[2] 游明鸿[2] 季晓菲[2] 张昌兵[2] 吴婍[2] 苟文龙[2] 李达旭[2] 陈仕勇[1] 白史且[2] 鄢家俊[2] LI Ming-feng;LI Xin-rui;LI Ying-zhu;CHEN Li-li;WANG Yan;LI Chun-yan;CHANG Dan;LEI Xiong;YAN Li-jun;YOU Ming-hong;JI Xiao-fei;ZHANG Chang-bing;WU Qi;GOU Wen-long;LI Da-xu;CHEN Shi-yong;BAI Shi-qie;YAN Jia-jun(Southwest Minzu University,Chengdu,Sichuan Province 610041,China;Sichuan Academy of Grassland Sciences,Chengdu,Sichuan Province 611743,China;Sichuan Agricultural University,Chengdu,Sichuan Province 611130,China)

机构地区：[1]西南民族大学,四川成都610041 [2]四川省草原科学研究院,四川成都611743 [3]四川农业大学,四川成都611130

出　　处：《草地学报》2023年第4期1026-1035,共10页Acta Agrestia Sinica

基　　金：四川省科技创新人才项目——青藏高原乡土草种老芒麦种质资源重要性状精准鉴定与育种应用(2023JDRC0040);四川省林草科技创新团队专项资金(LCTD2023CZ01);四川省“十四五”饲草育种攻关—高寒牧区多用途牧草新品种选育及配套技术研究(2021YFYZ0013-2);国家种质资源红原观测实验站(NAES020GR04);野生植物四川省科技资源共享服务平台资助。

摘　　要：为更好地利用老芒麦(Elymus sibiricus L.)野生资源,本研究从我国青藏高原、西北、华北、东北地区以及国外部分地区共采集了1723份野生披碱草属种质资源,通过表型特征观测和流式细胞仪检测,共鉴定出了990份野生老芒麦种质,246份垂穗披碱草种质,并测得老芒麦的DNA含量在5.86~7.30 Gb之间,平均DNA含量为6.66 Gb;垂穗披碱草材料的DNA含量在9.50~10.36 Gb之间,平均DNA含量为9.97 Gb。研究结果显示,在西北、华北以及东北地区的部分野生老芒麦材料在苗期基部叶鞘出现了绒毛,分析发现该表型出现的概率与经度和纬度呈极显著正相关关系(P<0.01),与海拔、年年均气温以及年平均降雨量呈极显著负相关关系(P<0.01)。表明老芒麦苗期基部叶鞘绒毛的有无与环境因子之间存在着必然的联系,可能是老芒麦种质应对不同环境类型的适应性机制之一。本研究为老芒麦的形态鉴定提供了新的依据,为老芒麦种质资源挖掘和育种应用提供了材料基础。In order to make better use of the wild resources of Elymus sibiricus germplasm,in this study the 1723 materials of wild Elymus species resources from the Qinghai-Tibet Plateau,Northwest of China,North China and Northeast of China and some foreign regions were collected,from which,total of 990 wild E.sibiricus germplasm were identified by phenotypic characteristics and the Flow Cytometry detection.The results showed that the DNA content of E.sibiricus was between 5.86~7.30 Gb with average of 6.66Gb.The DNA content of Elymus nutans Griseb was between 9.50~10.36 Gb with average of 9.97Gb.It was found out that dorsal hair appeared in the basal leaf sheath of some wild E.sibiricus materials from Northwest of China,North China and Northeast of China,which was different from the description of the morphological characteristics of E.sibiricus in Chinese Flora,which was positively correlated with longitude and latitude(P<0.01),and negatively correlated with altitude,annual mean temperature and annual mean rainfall(P<0.01),indicating that there was an inevitable relationship between the existence of the dorsal hair in the basal leaf sheath of E.sibiricus and its environmental factors and which possibly was one of the adaptive mechanisms of E.sibiricus germplasm to its environment.The results of this study provided a new basis for the morphological identification of E.sibiricus,and for the exploring E.sibiricus germplasm resources and breeding application.

关 键 词：老芒麦 表型鉴定 流式细胞术 基部叶鞘绒毛 

分 类 号：S543.9[农业科学—作物学]