SSR荧光标记毛细管电泳分析24个广西常规香稻品种的遗传多样性  

Genetic Diversity Analysis of 24 Guangxi Common Aromatic Rice Varieties by Fluorescent SSR Marker Capillary Electrophoresis

作　　者：唐梅 孙富[1,2] 何聪 卢宏琮[1,2] 黄鹂 夏秀忠[1] 唐忠平 钟志坚[3] 陆桂耀 TANG Mei;SUN Fu;HE Cong;LU Hongcong;HUANG Li;XIA Xiuzhong;TANG Zhongping;ZHONG Zhijian;LU Guiyao(Rice Research Institute,Guangxi Academy of Agricultural Sciences/Guangxi Key Laboratory of Rice Genetics and Breeding/Guangxi Talent Base for Rice Breeding Research,Nanning,Guangxi 530007,China;Seed Quality Testing Center,Guangxi Academy of Agricultural Sciences,Nanning,Guangxi 530007,China;Guangxi Seed Association,Nanning,Guangxi 530022,China)

机构地区：[1]广西壮族自治区农业科学院水稻研究所/广西水稻遗传育种重点实验室/广西水稻优质化育种研究人才小高地,广西南宁530007 [2]广西壮族自治区农业科学院种子质量检测中心,广西南宁530007 [3]广西种子管理站,广西南宁530022

出　　处：《热带作物学报》2023年第3期506-515,共10页Chinese Journal of Tropical Crops

基　　金：广西科技基地和人才专项(桂科AD20159088);广西农业科学院基本科研业务专项(桂农科2020YM71);广西农业科学院稳定资助科研团队项目(桂农科2021YT034)。

摘　　要：分析香稻品种的遗传多样性和理清香稻品种之间的亲缘关系,可为选育优质香稻品种提供参考。为分析广西审定的24个常规香稻品种的遗传多样性,明确其遗传差异,本研究利用SSR荧光标记毛细管电泳技术对24个广西常规香稻品种进行遗传多样性分析。结果表明,48对SSR引物共检测出170个等位基因位点;遗传多样性参数中观测等位基因数(Na)变化幅度为1~9,平均为3.54;有效等位基因数(Ne)的变化幅度为1~4.5534,平均为2.0736,其中有效等位基因数最高是RM21,其次是RM481、RM493和RM258,说明这4对引物有较高的检测效率;Shannon’s指数(I)的变化幅度为0~1.7942,平均为0.7726;多态信息含量(PIC)值的变化幅度为0~0.7541,平均为0.3732,属于中度多态位点,说明24个广西常规香稻品种具有一定的遗传变异。24个品种遗传相似系数范围为0.3299~0.9600,其中‘三香628’与‘农香32’遗传相似系数最小,说明这2个品种遗传基础差异最大,亲缘关系最远,‘万香696’与‘广粮香2号’遗传相似性系数最大,说明这2个品种遗传基础差异较小,亲缘关系接近。聚类分析结果显示24个广西常规香稻品种在相似系数0.504处可以分为3个大类群和4个亚类群,第Ⅰ大类2个品种,第Ⅱ大类19个品种,第Ⅲ大类3个品种;第Ⅱ大类在相似系数0.624处可分为4个亚类,第ⅰ亚类1个品种,第ⅱ亚类14个品种,第ⅲ亚类3个品种,第ⅳ亚类1个品种。研究结果表明24个广西常规香稻品种具有一定的遗传多样性,但不够丰富。以数字编码形式构建24个广西常规香稻品种的DNA指纹图谱。其分析结果可为广西常规香稻品种选育和鉴定提供参考。The analysis of the genetic diversity of aromatic rice varieties and clarification of the genetic relationship of aromatic rice varieties can provide references for breeding high-quality aromatic rice varieties.24 conventional aromatic rice varieties approved in Guangxi was used in the study.The genetic diversity of the varieties was analyzed by SSR fluorescence labeled capillary electrophoresis in this study.The results showed that a total of 170 alleles were detected by 48 SSR primers.The number of alleles(Na)varied from 1 to 9,with an average of 3.54.The range of effective allele number(Ne)ranged from 1 to 4.5534,with an average of 2.0736.The highest effective allele number was RM21,fol-lowed by RM481,RM493 and RM258.Four pairs of primers had high detection efficiency.Shannon's index(I)ranged from 0 to 1.7942 with an average of 0.7726.The variation range of polymorphic information content(PIC)ranged from 0 to 0.7541 with an average value of 0.3732,indicating that the 24 varieties had certain genetic variation.The genetic similarity coefficient of the 24 varieties ranged from 0.3299 to 0.9600,among which the genetic similarity coefficient of‘Sanxiang 628’and‘Nongxiang 32’was the lowest,indicating that the genetic basis difference of the two varieties was the greatest and the genetic relationship was the furthest.The genetic similarity coefficient of‘Wanxiang 696’and‘Guangliangxiang 2’was the greatest,indicating that the genetic basis difference of the two varieties was small and the genetic relationship was close.Cluster analysis showed that the 24 cultivars could be divided into three large groups and four subgroups at the similarity coefficient of 0.504.There were two varieties in theⅠcategory,19 varieties in theⅡcategory and 3 varieties in theⅢcategory.CategoryⅡcould be divided into 4 subclasses at the similarity coefficient of 0.624,including 1 variety in Subclassⅰ,14 varieties in subclassⅱ,3 varieties in subclassⅲ,and 1 variety in sub-classⅳ.The results showed that there was

关 键 词：广西 常规香稻 SSR荧光标记 毛细管电泳 遗传多样性 

分 类 号：S511[农业科学—作物学]