甘蔗植株茎部内生细菌群落组成对不同配方缓释肥的响应  

Response of Endophytic Bacterial Community Compositions in Stems of Sugarcanes under Different Slow-release Fertilizer Applications

作　　者：肖健 梁阗[2] 杨尚东[1] 谭宏伟[2] XIAO Jian;LIANG Tian;YANG Shangdong;TAN Hongwei(College of Agriculture,Guangxi University,Nanning,Guangxi 530004,China;Guangxi Academy of Agricultural Sciences/Guangxi Key Laboratory of Sugarcane Genetic Improvement,Nanning,Guangxi 530007,China;Longping Branch,College of Biology,Hunan University,Changsha,Hunan 410125)

机构地区：[1]广西大学农学院,广西南宁530004 [2]广西农业科学院/广西甘蔗遗传改良重点实验室,广西南宁530007 [3]湖南大学生物学院隆平分院,湖南长沙410125

出　　处：《热带作物学报》2023年第3期614-627,共14页Chinese Journal of Tropical Crops

基　　金：国家重点研发计划项目(No.2020YFD10006005);FAO项目(FAO/CPR/3804);国家糖料产业技术体系岗位(甘蔗宿根栽培)2017-2020(No.CARS170206)。

摘　　要：解析甘蔗植株茎部内生细菌群落组成对不同缓释肥的响应,旨在筛选科学的甘蔗专用缓释肥配方与用量,为进一步推广应用缓释肥提供理论依据和技术支撑。采用高通量测序技术,分析常规施肥(CK)和不同配方缓释肥处理(A~F)甘蔗植株茎部内生细菌群落组成变化。与CK相比,添加长效剂(235 g/t)和增效剂(3 g/t和18 g/t)的缓释肥处理均显著提高甘蔗植株茎部内生细菌的多样性,且除添加长效剂的缓释肥C处理(235 g/t)之外,施用其他配方缓释肥未能提升植株茎部内生细菌的丰富度;草螺菌属(Herbaspirillum)细菌是CK处理甘蔗植株茎部特有的优势内生细菌属;生孢噬纤维菌属(Sporocytophaga)和纤维弧菌属(Cellvibrio)是添加长效剂缓释肥A处理(125 g/t)甘蔗植株茎部特有的优势内生细菌属;添加长效剂的缓释肥B处理(150 g/t)甘蔗植株茎部未发现特有的优势内生细菌属;norank_f__SC-I-84、norank_f__Neisseriaceae、norank_f__JG30-KF-AS9和马赛菌属(Massilia)细菌是添加长效剂缓释肥C处理(235 g/t)甘蔗植株茎部特有的优势内生细菌属;短波单胞菌属(Brevundimonas)、肠杆菌属(Enterobacter)和葡萄球菌属(Staphylococcus)细菌是添加增效剂缓释肥D处理(3 g/t)甘蔗植株茎部特有的优势内生细菌属;克劳斯氏菌属(Cloacibacterium)细菌是添加增效剂缓释肥E处理(8 g/t)甘蔗植株茎部特有的优势内生细菌属;unclassified_f__Burkholderiaceae、类芽孢杆菌属(Paenibacillus)、异样根瘤菌属(Allorhizobium-Neorhizobium-Pararhizobium-Rhizobium)、Corynebacterium_1和金黄杆菌属(Chryseobacterium)细菌是添加增效剂缓释肥F处理(18 g/t)甘蔗植株茎部特有的优势内生细菌属。基因功能预测结果显示,施用不同配方缓释肥处理没有显著改变甘蔗植株茎部内生细菌的主体功能。与CK相比,施用添加长效剂(235 g/t),以及增效剂(3 g/t和18 g/t)用量的缓释肥处理能显著提高甘蔗植株茎部内生细菌�To select suitable slow-release fertilizers for sugarcane productions the endophytic bacterial community structure in the stems of sugarcane under different slow-release fertilizer applications were analyzed.Based on high-thr-oughput sequencing techniques,the endophytic bacterial community structure in the stems of sugarcanes among con-ventional fertilization(CK)and different slow-release fertilizers(A-F treatments)were analyzed.In comparison with CK,the diversity of the endophytic bacteria in the stems of sugarcanes was significantly increased by adding long-act-ing agent(235 g/t)and synergist(3 g/t and 18 g/t),but the richness of the endophytic bacteria in the stems of sugarcanes was not significantly improved by slow-release fertilizer applications except adding long-acting agent(235 g/t)treat-ment C.Meanwhile,Herbaspirillum was the specific endophytic dominant bacterial genus in CK.Sporocytophaga and Cellvibrio were the unique endophytic dominant bacterial genera in the slow-release fertilizer adding long-acting agent(125 g/t)treatment A.However,no specific endophytic dominant bacterial genera could be found in the slow-release fertilizer adding long-acting agent(150 g/t)treatment B.Norank_f__SC-I-84,norank_f__Neisseriaceae,no-rank_f__JG30-KF-AS9 and Massilia were the specific endophytic dominant bacterial genera in the slow-release fertil-izer adding long-acting agent(235 g/t)treatment C.Brevundimonas,Enterobacter,Staphylococcus were the unique endophytic dominant bacterial genera in the slow-release fertilizer adding synergist(3 g/t)treatment D.Cloacibacterium was the specific endophytic dominant bacterial genus in the slow-release fertilizer adding synergist(8 g/t)treatment E.And unclassified_f__Burkholderiaceae,Paenibacillus,Allorhizobium-Neorhizobium-Pararhizobium-Rhizobium,Cory-nebacterium_1,Chryseobacterium were the specific endophytic dominant bacterial genera in the slow-release fertilizer of adding synergist(18 g/t)treatment F.Furthermore,the functions of the endophytic bacteria in the stems of suga

关 键 词：甘蔗 缓释肥 内生细菌 高通量测序 

分 类 号：S566.1[农业科学—作物学]