Network pharmacology-based screening of the active ingredients and mechanisms of Cymbaria daurica against diabetes mellitus  

作　　者：Ruyu Shi Dongxue Chen Mingyue Ji Baochang Zhou Ziyan Zhang Chunhong Zhang Minhui Li 

机构地区：[1]Inner Mongolia Hospital of Traditional Chinese Medicine,Hohhot 010020,China [2]Inner Mongolia Key Laboratory of Characteristic Geoherbs Resources Protection and Utilization,Baotou Medical College,Baotou 014040,China [3]Inner Mongolia Institute of Traditional Chinese Medicine,Hohhot 010020,China [4]Inner Mongolia Engineering Research Center of the Planting and Development of Astragalus membranaceus of the Geoherbs,Baotou Medical College,Baotou 014040,China [5]Inner Mongolia Key Laboratory of Chinese Medicine Resources,Baotou Medical College,Baotou 014040,China

出　　处：《Food Science and Human Wellness》2023年第6期2001-2013,共13页食品科学与人类健康（英文）

基　　金：funded by the National Natural Science Foundation of China(81760776);the Natural Science Foundation of Inner Mongolia Autonomous Region(2018ZD13,2018LH08082,2019MS08208).

摘　　要：Cymbaria daurica L.has a long history as a folk medicine and tea for the treatment of diabetes.However,the biological activity and mechanism of its hypoglycemic effect have not been fully elucidated.In this study,the potential mechanism of C.daurica against type 2 diabetes mellitus(T2DM)was postulated via pharmacological network analysis.Based on data mining techniques involving topological parameters,gene ontology,and pathway enrichment,we established a compound-target,protein-protein interaction,and target-pathway network to identify central targets and pathways.Pathway enrichment analysis revealed that the most important pathway associated with C.daurica in treating T2DM is the PI3K-Akt signaling pathway.Molecular docking was performed to validate the predicted results.Then,a HepG2 cell insulin resistance model and a high-fat,high-glucose diet combined with a streptozotocin-induced T2DM rat model was established to assess the fasting glucose changes and lipid profile after C.daurica treatment,respectively.Finally,real-time PCR and western blotting were used to verify changes in key targets.The anti-diabetic mechanism of the active ingredient in C.daurica may involve the regulation of IRS-2,Akt1,GLUT4,and GSK3β.

关 键 词：Cymbaria daurica L.Diabetes mellitus Network pharmacology Insulin resistance Molecular docking PI3K-Akt signaling pathway 

分 类 号：R285[医药卫生—中药学]