Ginsenoside F1 administration promotes UCP1-dependent fat browning and ameliorates obesity-associated insulin resistance  

作　　者：Yuhan Meng Weili Li Chenxing Hu Si Chen Haiyang Li Feifei Bai Lujuan Zheng Ye Yuan Yuying Fan Yifa Zhou 

机构地区：[1]Engineering Research Center of Glycoconjugates of Ministry of Education,Jilin Provincial Key Laboratory of Chemistry and Biology of Changbai Mountain Natural Drugs,School of Life Sciences,Northeast Normal University,Changchun 130024,China

出　　处：《Food Science and Human Wellness》2023年第6期2061-2072,共12页食品科学与人类健康（英文）

基　　金：supported by the National Natural Science Foundation of China[31872674];the Jilin Talent Development Foundation Grant[20200301018RQ];the Fundamental Research Funds for the Central Universities[CGZH202206].

摘　　要：Obesity-induced type 2 diabetes is mainly due to excessive free fatty acids leading to insulin resistance.Increasing thermogenesis is regarded as an effective strategy for hypolipidemia and hypoglycemia.Ginsenoside is a natural active component in Panax ginseng C.A.Meyer,and some of them enhance thermogenesis.However,there are few studies on the mechanism and target of ginsenosides enhancing thermogenesis.Using thermogenic protein uncoupling protein 1(UCP1)-luciferase reporter assay,we identifi ed ginsenoside F1 as a novel UCP1 activator in the ginsenosides library.Using pull down assay and inhibitor interference,we found F1 binds toβ3-adrenergic receptors(β3-AR)to enhance UCP1 expression via cAMP/PKA/CREB pathway.We also investigated the ability of F1 on energy metabolism in obesity-induced diabetic mice,including body weight,body composition and energy expenditure.The results of proteomics showed that F1 signifi cantly up-regulated thermogenesis proteins and lipolytic proteins,but down-regulated fatty acid synthesis proteins.Ginsenoside F1 increased thermogenesis and ameliorated insulin resistance specifi cally by promoting the browning of white adipose tissue in obese mice.Additionally,ginsenoside F1 improves norepinephrine-induced insulin resistance in adipocytes and hepatocytes,and shows a stronger mitochondria respiration ability than norepinephrine.These fi ndings suggest that ginsenoside F1 is a promising lead compound in the improvement of insulin resistance.

关 键 词：Ginsenoside F1 Uncoupling protein 1 β3-Adrenergic receptor White adipose tissue browning Insulin resistance 

分 类 号：TQ460.1[化学工程—制药化工]