Fabrication of taxifolin loaded zein-caseinate nanoparticles and its bioavailability in rats  

作　　者：Ying Li Hang Su Wenjun Wang Zhongping Yin Jing’en Li En Yuan Qingfeng Zhang 

机构地区：[1]Jiangxi Key Laboratory of Natural Product and Functional Food,College of Food Science and Engineering,Jiangxi Agricultural University,Nanchang 330045,China [2]College of Pharmacy,Jiangxi University of Traditional Chinese Medicine,Nanchang 330006,China

出　　处：《Food Science and Human Wellness》2023年第6期2306-2313,共8页食品科学与人类健康（英文）

基　　金：supported by the National Natural Science Foundation of China(32060541).

摘　　要：Taxifolin loaded zein-caseinate nanoparticles(TZP)were fabricated by the anti-solvent method and were used as an oral delivery vehicle to improve their bioavailability in the rat.The formulations of TZP were optimized.With mass ratio of 1:1:2 between taxifolin,zein and sodium caseinate,the particle size andζpotential of TZP were(168.74±0.35)nm and−(57.67±0.25)mV,while the encapsulation and loading efficiency of taxifolin were(85.83±0.89)%and(17.11±0.88)%,respectively.After freeze-drying,TZP exhibited excellent redispersibility in water without aggregation.Physicochemical characterization showed that taxifolin existed in amorphous form in TZP and its interaction with the protein was observed.After encapsulating in TZP,the excellent dispersion of taxifolin in water signifi cantly improve its diffusion velocity through a semipermeable membrane.After oral administration,taxifolin and its 5 metabolites were identifi ed in rat plasma by ultra high performance liquid chromatography(UPLC)with quadrupole time-of-flight mass spectrometry(UPLC-QTOF-MS).The dynamic variation of taxifolin and its metabolites in plasma were then quantifi ed by UPLC with a triple-quadrupole typemass spectroscopy(UPLC-QqQ-MS/MS).A pharmacokinetic study showed that the bioavailability of taxifolin increased from 0.35%to 0.52%through TZP fabrication.The plasma concentration of taxifolin glucuronide and methylated taxifolin glucuronide was much higher than taxifolin.Glucuronidation was the dominating metabolism pathway of taxifolin in vivo.

关 键 词：TAXIFOLIN Zein nanoparticles Characterization BIOAVAILABILITY 

分 类 号：TB383.1[一般工业技术—材料科学与工程] TQ460.4[化学工程—制药化工]