Tapered optical fiber DNA biosensor for detecting Leptospira DNA  

作　　者：Jia-Yong Lam Mohd Hanif Yaacob Hui-Yee Chee 

机构地区：[1]Department of Medical Microbiology,Faculty of Medicine and Health Sciences,Universiti Putra Malaysia,43400 UPM Serdang,Selangor,Malaysia [2]Department of Computer and Communication Systems Engineering,Faculty of Engineering,Universiti Putra Malaysia,43400 UPM Serdang,Selangor,Malaysia [3]Wireless and Photonics Networks Research Centre(WiPNET),Universiti Putra Malaysia,43400 UPM Serdang,Selangor,Malaysia

出　　处：《Asian Pacific Journal of Tropical Medicine》2023年第3期119-128,共10页亚太热带医药杂志（英文版）

基　　金：funded by Universiti Putra Malaysia through the Geran Inisiatif Putra Siswazah (GP-IPS/2019/9678200)。

摘　　要：Objective: To establish a DNA detection platform based on a tapered optical fiber to detect Leptospira DNA by targeting the leptospiral secY gene.Methods: The biosensor works on the principle of light propagating in the special geometry of the optical fiber tapered from a waist diameter of 125 to 12 μm. The fiber surface was functionalized through a cascade of chemical treatments and the immobilization of a DNA capture probe targeting the secY gene. The presence of the target DNA was determined from the wavelength shift in the optical transmission spectrum.Results: The biosensor demonstrated good sensitivity, detecting Leptospira DNA at 0.001 ng/μL, and was selective for Leptospira DNA without cross-reactivity with non-leptospiral microorganisms. The biosensor specifically detected DNA that was specifically amplified through the loop-mediated isothermal amplification approach.Conclusions: These findings warrant the potential of this platform to be developed as a novel alternative approach to diagnose leptospirosis.

关 键 词：DNA biosensor Tapered optical fiber LEPTOSPIROSIS LEPTOSPIRA 

分 类 号：R377.5[医药卫生—病原生物学]