可溶性识别分子PTX3在ST段抬高型心肌梗死中的作用及其分子机制的研究  

Role of soluble recognition molecule PTX3 in ST segment elevation myocardial infarction and its molecular mechanism

作　　者：苏艳 孙倩[1] 张雅兰 李晓琳[1] Su Yan;Sun Qian;Zhang Yalan;Li Xiaolin(Department of Cardiovascular Medicine,the No.2 Hospital of Baoding,Baoding Hebei 071051,China;Special Needs Clinic,the No.2 Hospital of Baoding,Baoding Hebei 071051,China)

机构地区：[1]保定市第二医院心血管内一科,河北保定071051 [2]保定市第二医院特需诊疗科,河北保定071051

出　　处：《遵义医科大学学报》2023年第4期403-408,共6页Journal of Zunyi Medical University

基　　金：保定市科技计划项目(NO:2141ZF009)。

摘　　要：目的探究可溶性识别分子pentraxin 3(PTX3)在急性ST段抬高型心肌梗死中的表达水平及其作用机制。方法临床实验选择临床资料完整的STEMI患者93例以及183例健康对照,收集两组样本的血样,通过生化仪分别测定SOD和炎症分子IL-1β、IL-6,TNF-α表达水平;同时通过qPCR检测外周血细胞PTX3表达水平,细胞学实验:将人心肌细胞分为3组,对照组(不转染)、NC组(转染NC质粒)和试验组(转染PTX3质粒),通过qPCR检测细胞中炎症因子IL-1β、IL-6,TNF-α表达水平,通过流式细胞检测各组细胞凋亡水平。结果与对照组相比,STEMI组患者PTX3高表达,血清炎症因子IL-1β、IL-6、TNF-α表达水平显著升高,差异具有统计学意义(P<0.05);细胞学实验表明,与对照组相比,试验组细胞炎症因子IL-1β、IL-6、TNF-α表达水平显著升高,细胞凋亡比例显著增加,差异具有统计学意义(P<0.05)。结论STEMI患者中PTX3表达水平显著升高,炎症因子分泌增加,PTX3可能通过促进炎症因子的分泌诱导心肌细胞的凋亡,引发心肌损伤。Objective To investigate the expression level and mechanism of soluble recognition molecule pentraxin 3(PTX3)in acute ST segment elevation myocardial infarction(STEMI).Methods 93 patients with STEMI and 183 healthy controls with complete clinical data obtained in Baoding second hospital from January 2020 to January 2022 were selected for the clinical experiment.The blood samples of the two groups were collected,and the SOD and inflammatory molecule IL-1β、IL-6,TNF-αwere detected by biochemical instrument,the expression level of PTX3 in peripheral blood cells was detected by qPCR.Cytological experiment:human cardiomyocytes(HCM)were divided into three groups:control group(not transfected),NC group(transfected with NC plasmid)and test group(transfected with PTX3 plasmid).The inflammatory factor IL-1β、IL-6,TNF-αin cells was detected by qPCR.The level of apoptosis was detected by flow cytometry.Results compared with the control group,patients in STEMI group had higher expression of PTX3 and serum inflammatory factor IL-1β、IL-6、TNF-αThe expression level increased significantly,and the difference was statistically significant(P<0.05);Cytological experiments showed that compared with the control group,the inflammatory factor IL-1β、IL-6、TNF-αincreased in the experimental group;The expression level and the proportion of apoptosis increased significantly(P<0.05).Conclusion The expression level of PTX3 is significantly increased and the secretion of inflammatory factors is increased in patients with STEMI.PTX3 may induce cardiomyocyte apoptosis and cause myocardial injury by promoting the secretion of inflammatory factors.

关 键 词：ST段抬高型心肌梗死 PTX3 炎症因子 凋亡 损伤 

分 类 号：R392.7[医药卫生—免疫学]