乌拉草提取物对大鼠皮肤烫伤后金黄色葡萄球菌感染的抑菌作用  

Becteriostatic effect of Carex meyeriana Kunth extract on infection of Staphylococcus aureus after skin scalding in rats

作　　者：孙宏宇 陈子涵 崔佰吉 冯宪敏 刘迪 SUN Hongyu;CHEN Zihan;CUI Baiji;FENG Xianmin;LIU Di(Department of Pathogeny Biology,School of Basic Medical Sciences,Jilin Medical University,Jilin 132013,China)

机构地区：[1]吉林医药学院基础医学院病原生物学教研室,吉林吉林132013

出　　处：《吉林大学学报（医学版）》2023年第2期377-384,共8页Journal of Jilin University：Medicine Edition

基　　金：吉林省卫健委卫生与健康青年科技骨干培养计划项目(2019Q031);吉林省教育厅科学技术研究项目(JJKH20210499KJ,JJKH20210481KJ);吉林省中医药管理局中医药科技项目(2019139)。

摘　　要：目的:探讨乌拉草提取物黄酮类成分对大鼠皮肤烫伤后金黄色葡萄球菌(S.aureus)感染的抑菌作用,为新型抗感染中药的研发提供新思路。方法:采用水醇提取法提取乌拉草有效成分并利用紫外分光光度法测定其黄酮含量,采用牛津杯法抑菌试验检测乌拉草有效成分对细菌的抑菌活性,试管稀释法测定其最低抑菌浓度(MIC)。24只SD大鼠采用自制烫伤仪构建Ⅱ度烫伤模型,随机分为生理盐水对照组、青霉素钠(NaBP)对照组和乌拉草提取物CM-70治疗组,每组8只。3组大鼠创面皮内多点注射接种20μL(1.0×10^(8)CFU·mL^(-1))S.aureus,次日3组大鼠分别涂抹无菌生理盐水(0.5 g无菌脱脂棉吸附生理盐水0.5 mL·cm^(-2))、NaBP(0.5 g无菌脱脂棉吸附NaBP,5000 U·kg^(-1)体质量)和10 g·L^(-1)乌拉草CM-70提取物稀释液(0.5 g无菌脱脂棉吸附0.5 mL·cm^(-2)乌拉草CM-70提取液)。观察各组大鼠创面感染情况,采用创面痂下细菌定量实验检测各组大鼠烫伤后第1、2、3、4、5、6和7天创面细菌数(CFU·g^(-1)),酶联免疫吸附试验(ELISA)法检测各组大鼠烫伤前和烫伤后第1、3、7及14天血清白细胞介素1β(IL-1β)、白细胞介素10(IL-10)和肿瘤坏死因子α(TNF-α)水平。结果:体外抑菌活性检测,不同稀释浓度乌拉草CM-70提取物稀释液针对金黄色葡萄球菌形成不同大小抑菌圈,针对大肠埃希菌和白色念珠菌未形成抑菌圈。MIC检测,乌拉草有效成分对S.aureus的MIC值为2.5 g·L^(-1)。各组大鼠烫伤创面感染和愈合情况观察,与生理盐水对照组比较,感染后第3天,乌拉草CM-70提取物治疗组大鼠伤口浆液渗出较少;感染后第7天,伤口浆液渗出减少;红肿减轻,伤口面积缩小;感染后第14天,伤口创面已完全干燥,无浆液渗出,表面形成痂皮,伤口愈合良好,与NaBP对照组相近。烫伤创面痂下细菌定量,与生理盐水对照组比较,同一时间点乌拉草CM-70提取物治疗组大鼠创面�Objective:To discuss the becteriostatic effect of flavonoids in the Carex meyeriana Kunth extract on infection of Staphylococcus aureus(S.aureus)of the rats after skin scalding,and to provide the new ideas,for the research on new-style anti-infection traditional Chinese medicine.Methods:The wateralcohol extraction method was used to extract the active ingredients of Carex meyeriana Kunth and the content of flavonoids was determined by UV spectrophotometry,Oxford cup bacteriostatic test was used to detect the bacteriostatic activity of active ingredients of the Carex meyeriana Kunth against bacteria,and its minimal inhibitory concentration(MIC)was determined by tube dilution method.A total of 24 SD rats were used to establishⅡdegree scalding models and randomly divided into normal saline control group,penicillin sodium(NaBP)control group and Carex meyeriana Kunth extract CM-70 treatment group,and there were 8 rats in each group.The rats in three groups were inoculated with 20μL(1.0×10^(8) CFU·mL^(-1))S.aureus by multi-point injection in the wound skin;on the next day,the rats in three groups were smeared with sterile normal saline(0.5 g sterile absorbent cotton adsorbed normal saline 0.5 mL·cm^(-2)),NaBP(0.5 g sterile absorbent cotton adsorbed NaBP,5000 U·kg^(-1) body mass)and 10 g·L^(-1) Carex meyeriana kunth CM-70 extract diluent(0.5 g sterile absorbent cotton adsorbed 0.5 mL·cm^(-2) Carex meyeriana Kunth extract CM-70 extract diluent).The wound infection of the rats in various groups were observed;the bacteria number(CFU·g^(-1))under scalding wound scab of the rats on the 1st,2nd,3rd,4th,5th,6th,and 7th days after scalding was measured by bacteria quantitative test;the serum levels of interleukin-1β(IL-1β),interleukin-10(IL-10),and tumor necrosis factor-α(TNF-α)of the rats in various groups before scalding and on the 1st,3rd,7th,and 14th days after scalding were detected by ELISA method.Results:The in vitro bacteriostatic activity test results showed that different dilution concentrations of Carex

关 键 词：乌拉草 皮肤烫伤 感染 金黄色葡萄球菌 炎症因子 抑菌作用 

分 类 号：R378.11[医药卫生—病原生物学]