肠道菌群代谢物脱氧胆酸对人脐带间充质干细胞hUC-MSCs增殖及细胞周期的影响  

Effect of Intestinal Flora Metabolites Deoxycholic Acid on the Proliferation and Cell Cycle of Human Umbilical Cord Mesenchymal Stem Cells

作　　者：梁彩红 孟明耀[3] 李欣欣 熊晶晶[1,2] 李檬 刘梅[1] 侯宗柳 黄永坤[1,2] LIANG Caihong;MENG Mingyao;LI Xinxin;XIONG Jingjing;LI Meng;LIU Mei;HOU Zongliu;HUANG Yongkun(Dept.of Pediatrics,The 1st Affiliated Hospital of Kunming Medical University,Kunming Yunnan 650032;Yunnan Provincial Key Laboratory of Laboratory Medicine,Kunming Yunnan 650032;Yan’an Hospital Affiliated to Kunming Medical University,Kunming Yunnan 650051,China)

机构地区：[1]昆明医科大学第一附属医院儿科,云南昆明650032 [2]云南省检验医学重点实验室,云南昆明650032 [3]昆明医科大学附属延安医院中心实验室,云南昆明650051

出　　处：《昆明医科大学学报》2023年第4期23-30,共8页Journal of Kunming Medical University

基　　金：云南省检验医学重点实验室开放课题基金资助项目(2017DG005)。

摘　　要：目的探讨肠道菌群代谢物次级胆汁酸DCA不同浓度和不同作用时间在体外环境下对人脐带间充质干细胞(human umbilical cord mesenchymal stem cells,hUC-MSCs)增殖和细胞周期的影响。方法CCK8及流式细胞仪检测在含不同浓度DCA(0.00µmol/L、1.56µmol/L、3.13µmol/L、6.25µmol/L、12.50µmol/L、25.00µmol/L、50.00µmol/L、100.00µmol/L、200.00µmol/L、400.00µmol/L、800.00µmol/L)干细胞培养基中培养hUC-MSCs 24 h、48 h、72 h后,干细胞增殖及细胞周期的变化。结果脱氧胆酸对hUC-MSCs增殖的影响体现在DCA剂量和作用时间两方面(两者交互作用,F=6.622,P<0.001);当浓度一定时,时间越长,细胞增殖越多;当作用时间一定时,浓度越高,细胞增殖抑制。浓度一定时,随时间的增加,细胞阻滞在G0/G1期的比例增加(P<0.001);不同作用时间DCA对hUC-MSCs的细胞周期表现为低浓度组细胞阻滞在G0/G1期和S期,高浓度组细胞阻滞在S期和G2/M期(P<0.001)。结论体外环境下,DCA对hUC-MSCs增殖及细胞周期的影响与浓度和作用时间相关。Objective To investigate the influence of intestinal flora metabolites secondary bile acid DCA at different concentration and reaction time on the proliferation and cell cycle of human umbilical cord mesenchymal stem cells(hUC-MSCs)in vitro.Methods CCK8 and flow cytometry were used to detect the changes in cell proliferation and cell cycle of hUC-MSCs cultured in stem cell medium containing different concentrations of DCA(0.00µmol/L,1.56µmol/L,3.13µmol/L,6.25µmol/L,12.50µmol/L,25.00µmol/L,50.00µmol/L,100.00µmol/L,200.00µmol/L,400.00µmol/L,800.00µmol/L)at 24h,48h and 72h.Results The effect of deoxycholic acid on the proliferation of hUC-MSCs was both dose-and time-dependent(interaction,F=6.622,P<0.001).When the concentration was constant,cell proliferation increases with time;When the treatment time was fixed,the cell proliferation decreased with the increase of concentration.When the concentration was constant,the proportion of cell was arrested in G0/G1 phase increased with time(P<0.001).The cell cycle of hUC-MSCs treated by DCA was blocked in G0/G1 and S phase in low concentration group,while S and G2/M phase in high concentration group(P<0.001).Conclusion The effects of DCA on the proliferation and cell cycle of hUC�MSCs were both dose and time in vitro.

关 键 词：肠道菌群 代谢物 脱氧胆酸 人脐带间充质干细胞 细胞增殖 细胞周期 

分 类 号：Q939.121[生物学—微生物学]