离体再生诱导创制多倍体桑树种质材料  

Regeneration in vitro Induction to Create Polyploid Mulberry Germplasm Materials

作　　者：李艳[1] 植爽 贾强强 黄熙浩 侯志伟 王茜龄[1] LI Yan;ZHI Shuang;JIA Qiang-qiang;HUANG Xi-hao;HOU Zhi-wei;WANG Xi-ling(College of Sericulture,Textile and Biomass Sciences,Southwest University,Chongqing 400715,China;Changdu Agricultural Science Research Institute of Tibet,Changdu Tibet Autonomous Region,854085,China)

机构地区：[1]西南大学蚕桑纺织与生物质科学学院,重庆400715 [2]西藏昌都市农业科学研究所,西藏昌都854085

出　　处：《蚕学通讯》2023年第1期1-7,共7页Newsletter of Sericultural Science

基　　金：重庆市商务委茧丝绸行业项目支持(20210611150932818);财政部和农业农村部:国家现代农业产业技术体系建设专项(CARS-18)。

摘　　要：建立稳定、高效的离体再生体系是创制桑树多倍体需要解决的关键技术之一。以野生桑树种质资源A07为材料,将其萌动的春芽作为外植体,于无菌环境下接种在添加3.0 mg/L 6-BA和0.2 mg/L NAA的MS培养基中,暗处理3 d后移至光照14 h/d、温度(25±2)℃的环境中培养,结果表明A07春芽外植体的污染率低,易再生且增殖率高、生长快。进一步将丛生芽用1.5 g/L秋水仙素溶液浸泡3 d,多倍体诱导率达到26.6%以上,再生丛生芽易生根,生根率高且根系壮,移栽成活率高。由此初步建立了野生桑树种质资源A07的组培快繁体系。Stable and efficient in vitro regeneration system is one of the key technologies to create mulberry polyploid.A07 was used as material and its germinating mulberry buds as explants,inoculated in a sterile environment in MS medium,supplemented with 3.0 mg/L 6-BA and 0.2 mg/L NAA,dark treatment for 3 days and then moved to light for 14 h/d,and cultured at temperature(25±2)℃.The results showed that A07 has a low pollution rate and is easy to regenerate.A07 has High proliferation rate and fast growth.The multiple buds were soaked with 0.15%colchicine solution for 3 days,and the induction rate was over 26.6%.The regeneration of the multiple buds is easy to take root,the rooting rate is high,the root is strong,and the transplant survival rate is high.The tissue culture rapid propagation system of wild mulberry germplasm resource A07 was preliminarily established.

关 键 词：桑种质资源 离体再生 秋水仙素 多倍体诱导 

分 类 号：S888.3[农业科学—特种经济动物饲养]