不同来源家蚕核型多角体病毒的致病力及其vp 39基因的遗传进化分析  

Pathogenicity and Genetic Evolution of vp 39 Gene of Bombyx mori Nuclear Polyhedrosis Virus from Different areas

作　　者：王霞 王平阳 崔秋英 蒋满贵 张雨丽 黄旭华 宋宪军 WANG Xia;Wang Ping-yang;CUI Qiu-ying;JIANG Man-gui;ZHANG Yu-li;HUANG Xu-hua;SONG Xian-jun(The General Extension Station of Sericulture Technology of Guangxi,Nanning 530007,China)

机构地区：[1]广西壮族自治区蚕业技术推广站,南宁530007

出　　处：《蚕学通讯》2023年第1期36-40,共5页Newsletter of Sericultural Science

基　　金：广西自然科学基金项目(2018GXNSFBA281005);广西桑蚕产业创新团队病虫害防治岗位(nycytxgxcxtd-2021-02-04)。

摘　　要：家蚕核型多角体病毒(BmNPV)是养蚕生产中的主要病原微生物之一。收集广西壮族自治区9个蚕区的BmNPV分离株,纯化后分别给家蚕品种两广二号的2龄起蚕添食感染,调查9个BmNPV分离株对2龄起蚕的LC_(50)在1.55×10^(6)~4.19×10^(6)mL^(-1)之间,各分离株间的致病力差异不显著。选择BmNPV的感染相关基因vp 39设计合成序列引物进行PCR,对各分离株的扩增产物测序并进行遗传进化分析,结果表明不同来源分离株的vp 39基因在进化树上归于一个分支上的几个层次分支,序列间的相似度大部分>98%,相互间的遗传距离也较小,部分<1.0,提示vp 39基因相对比较保守。研究结果为解析家蚕抗病毒机制提供了一些新的参考信息。Bombyx mori nuclear polyhedrosis virus(BmNPV)is one of the main pathogen in silkworm rearing.Nine kinds of BmNPV were collected and purified,then infected to the second larval silkworm of Liangguang No.2.The LC_(50)of the study among 9 areas were ranged from 1.55×10^(6)to 4.19×10^(6)polyhedron per Milliliter,and the difference was not significant.The phylogenetic tree BmNPV of different sources based on vp 39 which was amplificated,isolated and sequenced from 9 kinds of BmNPV,the results show that vp 39 gene was relatively conserved.BmNPV from different sources on the evolutionary tree were grouped in the same cluster,the sequence of BmNPV from 9 areas high genetic homology which most more than 98%,and short genetic distance which most less than 1.0.The results of this study provide new clues to the antiviral mechanism of Bombyx mori.

关 键 词：家蚕核型多角体病毒 分离株 致病力 vp 39基因 序列同源性 

分 类 号：S884[农业科学—特种经济动物饲养]