瑞替加滨抗惊厥及对惊厥性海马损伤保护效果的研究  

Anticonvulsant effect of retigabine and its protective effect on convulsive hippocampal injury

作　　者：徐静怡 张心雨 李连俊 刘阳 余建东 冯占辉 葛荣靖 XU Jingyi;ZHANG Xinyu;LI Lianjun(Department of Pathophysiology,Bengbu Medical College,Bengbu(233003),Anhui,China)

机构地区：[1]蚌埠医学院基础医学院病理生理学教研室,安徽蚌埠233003 [2]蚌埠医学院第一附属医院神经外科 [3]贵州医科大学附属医院神经内科

出　　处：《癫痫与神经电生理学杂志》2023年第2期70-75,共6页Journal of Epileptology and Electroneurophysiology(China)

基　　金：安徽省自然科学基金项目(2108085MC90);安徽省教育厅高校优秀人才支持计划重点项目(gxyqZD2021116)。

摘　　要：目的研究瑞替加滨(RTG)对急性惊厥的改善作用,并探究其对惊厥性海马损伤的保护效果。方法选取40只ICR雄性小鼠随机分为空白组(n=10)、惊厥组(n=10)、RTG组(n=10)及苯巴比妥钠组(n=10)。在小鼠急性惊厥发作过程中分别给予以上对症处理,通过分析小鼠惊厥发作等级观察RTG的抗惊厥效果;通过HE染色法检测小鼠海马组织的病理形态变化。将惊厥组小鼠麻醉取脑行全细胞电流钳记录海马齿状回区颗粒细胞的放电情况,灌流RTG观察其对颗粒细胞放电的影响。结果RTG可降低小鼠惊厥发作的等级和频次,苏木素-伊红染色结果显示RTG可显著改善小鼠海马CA1区惊厥后的神经元损伤(P<0.05)。RTG可降低惊厥小鼠海马齿状回区颗粒细胞的兴奋性,组间比较差异有统计学意义(P<0.05)。结论RTG可抑制小鼠惊厥,减轻小鼠海马惊厥性神经元损伤,并可减少小鼠惊厥后过度兴奋颗粒细胞的放电。Objective To investigate the anticonvulsant effect of retigabine(RTG)on acute convulsion and explore its protective effect on convulsive hippocampal injury.Methods Forty ICR male mice were randomly divided into control group(n=10),convulsion group(n=10),RTG group(n=10),and phenobarbital sodium group(n=10).The above symptomatic treatment were given in the course of acute seizures in mice,respectively.The seizure grades of mice were analyzed to observe the anticonvulsant effect of RTG.HE staining was applied to detect pathological morphology of mouse hippocampi.Whole cell patch current clamp technique was performed to record the discharge of granule cells in dentate gyri of the hippocampi in the brains collected from anesthetized mice in convulsion group.RTG was perfused to the brain slice during recording to observe its effects on granule cell's discharge.Results RTG reduced the grade and frequency of convulsion.HE staining results showed that RTG significantly improved neuronal damage in the CA1 region of mouse hippocampus after convulsion(P<0.05).RTG decreased the excitability of granule cells in the dentate gyri of convulsive mice(P<0.05).Conclusion RTG can inhibit mouse convulsion,alleviate neuronal damage in mouse hippocampus convulsion,and reduce the discharge of overexcited granular cells after mouse convulsion.

关 键 词：瑞替加滨 急性惊厥 海马 惊厥后神经元损伤 颗粒细胞 

分 类 号：R971.6[医药卫生—药品] R741[医药卫生—药学]