西藏濒危植物巨柏根际细菌群落特征  

Characteristics of the rhizosphere bacterial community of endangered plant Cupressus gigantea in Tibet

作　　者：巩文峰 王泽莹 刘金良[3] 孙玉 杨欣欣 魏帅 魏丽萍[2] GONG Wenfeng;WANG Zeying;LIU Jinliang;SUN Yu;YANG Xinxin;WEI Shuai;WEI Liping(College of Plant Sciences,Tibet Agricultural and Animal Husbandry University,Linzhi 860000,Xizang,China;College of Resources and Environment,Tibet Agricultural and Animal Husbandry University,Linzhi 860000,Xizang,China;College of Forestry,Northwest A&F University,Yangling 712100,Shaanxi,China)

机构地区：[1]西藏农牧学院植物科学学院,西藏林芝860000 [2]西藏农牧学院资源与环境学院,西藏林芝860000 [3]西北农林科技大学林学院,陕西杨凌712100

出　　处：《浙江大学学报（农业与生命科学版）》2023年第2期241-252,292,共13页Journal of Zhejiang University:Agriculture and Life Sciences

基　　金：西藏农牧学院林学学科创新团队建设项目(藏财预指2020-001);2022年度西北农林科技大学-西藏农牧学院科研联合基金项目(XNLH2022-02);西藏高原生态安全联合重点实验室研究生开放基金项目(STAQ-2021Y-11)。

摘　　要：根际微生物在植物生长和环境适应中起着重要的作用。为揭示西藏濒危珍稀植物巨柏根际细菌群落特征,通过16S rRNA基因扩增子高通量测序技术,并结合土壤化学特性,分析西藏林芝市巴宜区、米林县、朗县3个种群巨柏根际细菌的组成、多样性及影响因子。结果显示:巨柏根际细菌多样性丰富,其中优势菌门为放线菌门(Actinobacteriota)、变形菌门(Proteobacteria)和酸杆菌门(Acidobacteriota)。朗县、巴宜区和米林县巨柏根际细菌共有属为757个(65.83%),独有属分别为125个(10.87%)、39个(3.39%)和41个(3.56%);基于Bray-Curtis距离的非度量多维尺度的β多样性分析显示,不同种群间根际细菌群落差异显著。细菌群落的共现性网络正相关连接均在68%以上,表明群落间合作关系大于竞争关系。土壤中有效磷、全磷、铵态氮是驱动根际细菌群落结构变化的重要因子。为适应不同生长环境,各种群巨柏形成了特有的根际细菌群落。本研究结果可为探究根际微生物在巨柏生长和环境适应中的作用及保护巨柏植物资源提供参考。Rhizosphere microorganisms play an important role in plant growth and adaptation to the environment.In order to reveal the characteristics of rhizosphere bacterial community of endangered plant Cupressus gigantea in Tibet,we employed 16S rRNA gene high-throughput amplicon sequencing technology combined with soil chemical properties to examine the rhizosphere bacterial composition and diversity in Bayi District,Milin County,and Langxian County of Nyingchi City in Tibet and their influencing factors.The results showed that the diversity of bacteria in the rhizosphere of C.gigantea was rich,and the dominant bacterial phylum were Actinobacteriota,Proteobacteria,and Acidobacteriota.There were 757 shared genera(65.83%)of rhizosphere bacteria in C.gigantea in Langxian County,Bayi District,and Milin County,with 125(10.87%),39(3.39%)and 41(3.56%)unique genera,respectively,and theβ-diversity analysis of non-metric multidimensional scaling based on Bray-Curtis distance revealed significant differences among populations.The positive correlation of the co-occurrence network of bacterial communities was more than 68%,and the cooperative relationship was greater than the competitive relationship.Soil available phosphorus(AP),total phosphorus(TP),and ammonium nitrogen(NH4+-N)were important factors driving changes in the structure of rhizosphere bacterial community.In order to adapt to different growth environments,various groups of C.gigantea have formed unique rhizosphere bacterial communities.The above results can provide a reference for studying the role of rhizosphere microorganisms in the growth and environmental adaptation of C.gigantea and the protection of C.gigantea resources.

关 键 词：巨柏 根际细菌 群落结构 土壤化学特性 

分 类 号：Q938.1[生物学—微生物学] S791.41[农业科学—林木遗传育种]