HPLC法测定石斛类药材中烟酸和烟酰胺的含量  

Simultaneous Determination of Niacin and Nicotinamide in Dendrobium by HPLC

作　　者：王飞轩 廖扬振 廖天月 万晶琼 陈国霞 朱南 韩邦兴[4] 欧阳臻[1] Wang Feixuan;Liao Yangzhen;Liao Tianyue;Wan Jingqiong;Chen Guoxia;Zhu Nan;Han Bangxing;Ouyang Zhen(School of Pharmacy,Jiangsu University,Zhenjiang 212013,China;Nanjing Institute of Product Quality Inspection,Nanjing 210019,China;School of Food and Biological Engineering,Jiangsu University,Zhenjiang 212013,China;School of Biological and Pharmaceutical Engineering,West Anhui University,Lu'an 237000,China)

机构地区：[1]江苏大学药学院,江苏镇江212013 [2]南京市产品质量监督检验院,江苏南京210019 [3]江苏大学食品与生物工程学院,江苏镇江212013 [4]皖西学院生物与制药工程学院,安徽六安237000

出　　处：《中国野生植物资源》2023年第4期23-27,38,共6页Chinese Wild Plant Resources

基　　金：国家自然科学基金面上项目(81872961);中央本级重大增减支项目(2060302);安徽省石斛产业化开发协同创新项目。

摘　　要：目的:建立同时测定石斛类药材中烟酸和烟酰胺含量的HPLC法。方法:采用Supersil AQ C-_(18)柱(4.6 mm×250 mm,5μm),0.1%庚烷磺酸钠溶液(含7%甲醇和2%异丙醇,pH至2.1±0.1)为流动相,流速为1.0 mL/min,检测波长为261 nm。结果:烟酸和烟酰胺分别在0.002~0.040 mg/mL(R^(2)=0.9995)、0.006~0.120 mg/mL(R^(2)=0.9995)浓度范围内线性关系良好,平均回收率为99.68%和99.14%,RSD为1.91%和1.74%。结论:不同种石斛类药材中烟酸与烟酰胺的含量有差异,铜皮石斛中烟酸含量最高,流苏石斛中烟酰胺含量最高。该研究建立的方法简便、准确、重复性好,为石斛药材的品质评价及开发研究提供科学依据。Objective:To establish a high performance liquid chromatography(HPLC)method for the simultaneous determination of niacin and nicotinamide in Dendrobium.Methods:Supersil AQ C-_(18) column(4.6 mm×250 mm,5μm)was used with 0.1%heptane sodium sulfonate solution(containing 7%methanol and 2%isopropanol,pH 2.1±0.1)as the mobile phase.The flow rate was 1.0 mL/min and the detection wavelength was 261 nm.Results:The good linear relationship of niacin and nicotinamide was shown within the range of 0.002-0.040 mg/mL(R^(2)=0.9995),0.006-0.120 mg/mL(R^(2)=0.9995),respectively,the average recovery rate were 99.68%(RSD=1.91%)and 99.14%(RSD=1.74%).Conclusion:The contents of niacin and nicotinamide in different Dendrobium species were different,the content of niacin in D.moniliforme was the highest,while the content of nicotinamide in D.fimhriatum was the highest.The established method was simple,accurate and reproducible,which provided a scientific basis for the quality evaluation and development of Dendrobium.

关 键 词：石斛 烟酸 烟酰胺 高效液相色谱法 含量测定 

分 类 号：R932[医药卫生—生药学]