植物蛋白质O-糖基化修饰的研究进展  

Recent Progress on Protein O-Glycosylation in Plants

作　　者：张月倩 苏晓 张耿 张雪萍 李晓娟[1,2] ZHANG Yueqian;SU Xiao;ZHANG Geng;ZHANG Xueping;LI Xiaojuan(Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants,Ministry of Education,College of Bioscience and Bioengineering,Beijing Forestry University,Beijing 100083,China;Institute of Tree Development and Genome Editing,Beijing Forestry University,Beijing 100083,China)

机构地区：[1]北京林业大学生物科学与技术学院,林木花卉遗传育种教育部重点实验室,北京100083 [2]北京林业大学树木发育与基因编辑研究院,北京100083

出　　处：《中国细胞生物学学报》2023年第2期247-257,共11页Chinese Journal of Cell Biology

基　　金：国家自然科学基金(批准号:91954202);北京林业大学“杰出青年人才”培育计划(批准号:2019JQ03003)资助的课题。

摘　　要：糖基化是最主要的蛋白质翻译后修饰方式之一,主要有N-糖基化、O-糖基化和糖基磷脂酰肌醇锚定修饰三种类型。在植物细胞中,O-糖基化修饰广泛发生,它不仅参与蛋白质转录调节、信号转导,还与细胞壁合成等生物学过程紧密相关。在多种O-糖基化修饰类型中,O-N-乙酰氨基葡萄糖(O-GlcNAc)糖基化修饰结构独特、易于检测和表征,因此已经有许多相关技术实现了对其的表征。然而,其他类型O-糖基化修饰蛋白的结构和功能仍有待更全面的研究。该文综述了植物蛋白中不同类型O-糖基化修饰的相关研究进展,总结了植物O-糖基化修饰蛋白检测技术的优缺点,最后展望了这些技术在植物蛋白质O-糖基化修饰研究中的应用前景。Glycosylation,including N-glycosylation,O-glycosylation,and glycosylphosphatidylinositol anchoring,is one of the most important post-translational modifications of proteins.O-glycosylation occurs widely in plant cells.It is not only involved in regulating protein transcription and signal transduction,but also closely related to cell wall synthesis.Among various kinds of O-glycosylated modifications,O-N-acetylglucosamine(O-GlcNAc)glycosylation which has specific structure is easy to be detected and characterized.Therefore,many techniques have been developed to detect and characterize these proteins.However,the structure and function of other O-glycosylated proteins still remain to be further elucidated.Here,this article reviews the research progresses of different types of O-glycosylated modifications in plants.Moreover,it summarizes the advantages and disadvantages of different technologies.Finally,the challenges and future directions in studying O-glycosylated protein in plant biology are discussed.

关 键 词：O-糖基化修饰 植物蛋白 生物学功能 蛋白修饰表征 

分 类 号：Q943[生物学—植物学]