Profiling the effects of microwave-assisted and soxhlet extraction techniques on the physicochemical attributes of Moringa oleifera seed oil and proteins  

作　　者：Ngozi Maryann Nebolisa Chukwuebuka Emmanuel Umeyor Uchenna Eunice Ekpunobi Immaculeta Chikamnele Umeyor Festus Basden Okoye 

机构地区：[1]Nanomedicines and Drug Delivery Research Group,Department of Pharmaceutics and Pharmaceutical Technology,Faculty of Pharmaceutical Sciences,Nnamdi Azikiwe University,Awka,422001,Anambra State,Nigeria [2]Department of Pure and Industrial Chemistry,Faculty of Physical Sciences,Nnamdi Azikiwe University,Awka,422001,Anambra State,Nigeria c [3]Department of Pharmacognosy and Traditional Medicine,Faculty of Pharmaceutical Sciences,Nnamdi Azikiwe University,Awka,422001,Anambra State,Nigeria [4]Department of Pharmaceutical and Medicinal Chemistry,Faculty of Pharmaceutical Sciences,Nnamdi Azikiwe University,Awka,422001,Anambra State,Nigeria

出　　处：《Oil Crop Science》2023年第1期16-26,共11页中国油料作物学报(英文版）

基　　金：funded by International Foundation for Science(IFS)and Organisation for the Prohibition of Chemical Weapons(OPCW)research grant awarded to Dr.Chukwuebuka Emmanuel Umeyor in 2019(Grant number:I-2-F-6448-1).

摘　　要：There is a constant search for biomaterials from natural products like plants for food and industrial applications.The work embodied in this report aimed at investigating the effects of microwave-assisted and soxhlet extraction(MAE and SE) techniques on the functional physicochemical quality characteristics of Moringa oleifera seed oil and proteins extracts. M. oleifera seeds were ground to fine powders and oil was extracted by microwave-assisted and soxhlet extraction techniques using petroleum ether. Quality attributes including yield percent, moisture content,iodine, saponification, specific gravity, viscosity, p H, thiobarbituric acid, acid and peroxide values were measured. Mineral and vitamin contents, chemical/functional groups, fatty acid(FA) composition, and reducing power of the oil were evaluated. Metabolomics of protein extracted from the defatted powders were analyzed by nuclear magnetic resonance(NMR). M. oleifera oil from MAE and SE methods had good yield(34.25 ± 0.0%,28.75 ± 0.0%), low moisture content(0.008 ± 0.0%, 0.011 ± 0.0%), non-drying and unsaturated, moderately saponified, less dense(0.91 ± 0.01, 0.92 ± 0.02 g m L^(-1)), had Newtonian flow, were weakly acidic, showed good content of FAs, recorded strong potential for long shelf-life, showed stability against oxidative rancidity and enzymatic hydrolysis, had very rich deposits of micro-and macro-nutrients as well as water-soluble and lipidsoluble vitamins, and functional groups in the oil were reflective of its content of long-and medium-chain triglycerides(LCT and MCT). Monounsaturated and saturated fatty acids(MUFA and SFA) were detected and the oil has excellent ferric ion reducing power. NMR metabolomic assay revealed the presence of nine essential amino acids(EAAs) in the protein extract. MAE technique is a feasible and acceptable alternative for high throughput extraction of M. oleifera oil with high yield and excellent quality attributes. The study revealed that MAE did not impart any remarkable advantage(s) on the physicochemi

关 键 词：Moringa oleifera seed Oil Microwave-assisted extraction Soxhlet extraction Quality attributes GC-MS assay Metabolomics Reducing power 

分 类 号：TQ28[化学工程—有机化工]