绵羊GRM 1基因多态性及其与肉质性状的相关性  

Analysis of GRM1 gene polymorphism and its correlation with meat quality traits in sheep

作　　者：聂玮 孟科 荣轩 强浩 郭晨浩 陶毛孩 冯登侦[1] NIE Wei;MENG Ke;RONG Xuan;QIANG Hao;GUO Chenhao;TAO Maohai;FENG Dengzhen(College of Agriculture,Ningxia University,Yinchuan 750021,China)

机构地区：[1]宁夏大学农学院,宁夏银川750021

出　　处：《浙江农业学报》2023年第4期799-808,共10页Acta Agriculturae Zhejiangensis

基　　金：宁夏回族自治区科学基金(2020AAC03082);宁夏回族自治区农业育种专项(NXNYYZ20150101)。

摘　　要：为探究绵羊代谢型谷氨酸受体1基因(GRM 1)多态性及其对绵羊肉质性状的影响,以期为绵羊肉质性状选育提供有效的遗传分子标记。采用液相捕获测序技术对滩羊(T)、小尾寒羊(XH)、杜泊羊(D)3个绵羊群体共91只绵羊的GRM 1基因进行测序,筛选出2个多态位点rs403075278和rs415006419。利用飞行质谱检测技术对滩羊(T)、小尾寒羊(XH)、杜泊羊(D)和杜滩寒三元杂交羊(DTH)共30只绵羊进行基因分型,对筛选出的位点与肉质性状进行关联分析。结果显示:不同基因型绵羊的肉质性状存在显著差异,GRM 1基因rs415006419位点的CT基因型绵羊肾的质量显著(P<0.05)大于TT基因型,TT基因型绵羊肌肉的酪氨酸含量显著(P<0.05)高于CT基因型。rs403075278位点TT基因型绵羊的宰前活重、胴体重、净肉重、十二指肠长度,以及心、肺和肾的质量均极显著(P<0.01)大于CT基因型;TT基因型绵羊的背膘厚、净肉重、脾的质量、肌肉的脂肪、钙含量、苯丙氨酸和组氨酸含量显著(P<0.05)高于CT基因型;CT基因型绵羊的pH值、剪切力和失水率极显著(P<0.01)大于TT基因型;CT和TT基因型的其余屠宰性能、肉品质和肌肉营养成分含量均无显著差异(P>0.05)。以上结果表明,GRM 1基因可以作为绵羊肉质性状的候选基因,rs415006419和rs403075278位点可以作为绵羊生长发育和产肉性能的候选遗传标记。To investigate the polymorphism of metabotropic glutamate receptor 1 gene(GRM 1)in sheep and its effect on meat quality traits,with a view to providing effective genetic molecular markers for the breeding of sheep for meat performance.The GRM 1 gene was sequenced in 91 sheep from three sheep populations,Tan sheep(T),Little-tailed Han sheep(XH),and Dorper sheep(D),using liquid-phase capture sequencing technology.The genotypes of the genotyped loci were analyzed by flight mass spectrometry for the meat quality traits of the Tan sheep(T),Little-tailed Han sheep(XH),Dorper sheep(D)and Dutanhan sheep(DTH).The results showed that there were significant differences in meat quality traits among the different genotypes of sheep.Sheep kidney mass with CT genotype was significantly(P<0.05)greater than that with TT genotype at the rs415006419 locus of the GRM 1 gene,and tyrosine content in muscle of sheep with TT genotype was significantly(P<0.05)higher than that with CT genotype;Sheep live weight before slaughter,carcass weight,net meat weight,duodenal length,and heart,lung and kidney mass with TT genotype at locus rs403075278 of the GRM 1 gene were significantly(P<0.01)greater than those with the CT genotype,and backfat thickness,net meat weight,spleen mass,muscle fat,calcium content,phenylalanine and histidine content with TT genotype were significantly(P<0.05)higher than those with CT genotype.pH,shear and water loss with CT genotype were significantly(P<0.01)greater than those with TT genotype.The remaining slaughter performance,meat quality and muscle nutrient content were not significantly(P>0.05)different between the two genotypes.The above results suggest that GRM 1 gene could be used as a candidate gene for meat quality traits in sheep,rs415006419 and rs403075278 loci could be used as potential candidate genetic markers for sheep growth and development,and meat production performance.

关 键 词：绵羊 代谢型谷氨酸受体1 多态性 屠宰性能 肉质 营养物质 

分 类 号：S826[农业科学—畜牧学]