盐酸吉西他滨联合铁死亡诱导剂对PANC-1细胞的增殖抑制作用  

Inhibitory effects of gemcitabine hydrochloride combined with ferroptosis inducers on the proliferation of PANC-1 cells

作　　者：王吉荣 杨道秋 孙治国 韩涵 邓莉 WANG Jirong;YANG Daoqiu;SUN Zhiguo;HAN Han;DENG Li(Department of Pharmaceutics,School of Pharmacy,Naval Medical University,Shanghai 200433,China;Department of Dermatology,No.970 Hospital of Joint Logistics Support Force of the PLA,Yantai 264002,China;Jingzhong Medical District,General Hospital of the PLA,Beijing 100820,China)

机构地区：[1]海军军医大学药学系药剂学教研室,上海200433 [2]中国人民解放军联勤保障部队第九七〇医院皮肤科,山东烟台264002 [3]解放军总医院京中医疗区,北京100820

出　　处：《药学实践与服务》2023年第4期234-239,共6页Journal of Pharmaceutical Practice and Service

摘　　要：目的研究三种铁死亡诱导剂埃拉斯汀(Era)、柳氮磺胺吡啶(SASP)和青蒿琥酯(Art)与盐酸吉西他滨(hcGEM)单独或联合应用对人胰腺癌PANC-1细胞的抑制作用。方法采用CCK-8法检测不同浓度的Era、SASP、Art单独或联合hcGEM对PANC-1细胞的增殖抑制作用,并根据联合指数(CI)判断三种铁死亡诱导剂联合hcGEM对PANC-1细胞是否具有协同抑制作用。结果hcGEM与三种铁死亡诱导剂单独或联合应用均能显著抑制PANC-1细胞活性,并且这种抑制作用随着浓度增大而增强。hcGEM-Era 4∶1或1∶4联用组、hcGEM-SASP 1∶400联用组CI值小于1。hcGEM-Art 1∶4或1∶16联用组仅在一定浓度范围内CI值小于1。结论hcGEM与三种铁死亡诱导剂单独或联合应用对PANC-1细胞的增殖抑制作用呈现剂量依赖性。hcGEM与三种铁死亡诱导剂联用能协同抑制PANC-1细胞的增殖。Objective To study the effects of three ferroptosis inducers Erastin(Era),sulfasalazine(SASP)and artesunate(Art)alone or combined with gemcitabine hydrochloride(hcGEM)on the proliferation inhibition of Human pancreatic cell line PANC-1.Methods The CCK-8 method was used to detect the inhibitory effects of different concentrations of Era,SASP and Art alone or combined with hcGEM on the proliferation of PANC-1,and the combination index(CI)was used to judge whether three ferroptosis inducers combined with hcGEM had synergistic inhibitory effect on PANC-1.Results The three ferroptosis inducers and hcGEM alone or in combination could significantly inhibit the activity of PANC-1.The inhibitory effects were enhanced with the concentration increasing.The CI values of hcGEM-Era 4∶1 or 1∶4 combination group and hcGEM-SASP 1∶400 combination group were less than 1.The CI values of hcGEM-Art 1∶4 or 1∶16 combination group were less than 1 only within a certain concentration range.Conclusion The inhibitory effects of the three ferroptosis inducers and hcGEM alone or in combination were dose-dependent.The combination of hcGEM and three ferroptosis inducers could synergistically inhibit the proliferation of PANC-1.

关 键 词：铁死亡诱导剂 盐酸吉西他滨 胰腺癌 协同抑制 

分 类 号：R965[医药卫生—药理学]