基于网络药理学探讨苣荬菜治疗脓毒血症的化学成分及其作用机制  

Discussion on the chemical compositions and treatment mechanism of sepsis of Sonchus arvensis L.by network pharmacology

作　　者：胡冰悦 张欣康 岑金凤 吕重宁 路金才[1] 肖凯 HU Bingyue;ZHANG Xinkang;CEN Jinfeng;LV Chongning;LU Jincai;XIAO Kai(School of Chinese Materia Medica,Shenyang Pharmaceutical University,Shenyang 110016,China;Lab of Toxicology&Pharmacology,Faculty of Naval Medicine,Naval Medical University,Shanghai 200433,China)

机构地区：[1]沈阳药科大学中药学院,辽宁沈阳110016 [2]海军军医大学海军医学系防化医学教研室,上海200433

出　　处：《药学实践与服务》2023年第4期245-251,共7页Journal of Pharmaceutical Practice and Service

摘　　要：目的基于文献挖掘及网络药理学探究苣荬菜中化学成分治疗脓毒血症的潜在作用机制。方法以文献中化学成分为研究对象,借助Swiss Target Prediction数据库预测成分的潜在作用靶点;使用GeneCards、OMIM、TTD数据库筛选脓毒血症相关靶点;通过Venny 2.1.0获得苣荬菜成分与脓毒血症的共同靶点;采用Cytoscape软件构建“药物成分-疾病靶点”网络;运用STRING数据库构建蛋白质相互作用(PPI)网络;运用DAVID数据库进行GO和KEGG富集分析。结果获得苣荬菜中化学成分71个,药物作用靶点579个,疾病相关靶点3437个,交集靶点272个。GO功能富集条目1366个,包括分子功能(MF)245个,生物过程(BP)1002个,细胞组成(CC)119个;KEGG信号通路166个。结论苣荬菜的主要活性成分包括亚油酸、亚麻酸、油酸等,它们可能通过作用于TNF、AKT1、IL-6、IL-1β、TP53等靶点,调节类固醇、鞘脂等各种激素以及表皮因子、趋化因子的表达,产生抗炎作用进而发挥治疗脓毒血症的功效。Objective To explore the effective constituents from Sonchus arvensis L.and the potential mechanism in treating sepsis by network pharmacology.Methods The chemical ingredients reported in the literature were taken as research objects and Swiss Target Prediction database was used to collect the identify the potential targets of those ingredients.The GeneCards,OMIM and TTD databases were applied to screen the sepsis related molecular targets.The cross targets were obtained and used to construct the active ingredient-disease target network.In addition,the targets were also imported into STRING database to construct a PPI network.Finally,GO and KEGG enrichment analysis were performed on the target genes to predict the mechanism via DAVID database.Results 71 components from S.arvensis L.were obtained,which corresponded to 579 potential drug targets.There were 3437 related targets of sepsis.S.arvensis L.and sepsis shared 272 common targets.The results showed that 1366 terms were found by GO function enrichment,including 245 molecular functions(MF),1002 biological processes(BP),and 119 cell composition(CC),The KEGG enrichment analysis suggested that 166 signaling pathways were involved.Conclusion The study revealed that TNF,AKT1,IL-6,IL-1β,TP53 and some other targets might be affected by potentially active ingredients of S arvensis L.such as linoleic acid,linolenic acid and oleic acid to regulate the expression of steroids,sphingolipids hormones as well as epidermal factors and chemokines in treating sepsis.

关 键 词：苣荬菜 脓毒血症 网络药理学 白细胞介素-6 肿瘤坏死因子 

分 类 号：R285[医药卫生—中药学]