Zwitterionic betaines over HEPES as the new generation biocompatible pH buffers for cell culture  

作　　者：Peiming Liu Jin Sun Wan Peng Yahui Gu Xiaoxue Ji Zhi Su Pingsheng Liu Jian Shen 

机构地区：[1]Jiangsu Collaborative Innovation Center of Biomedical Functional Materials,Jiangsu Key Laboratory of Bio-functional Materials,School of Chemistry and Materials Science,Nanjing Normal University,Jiangsu,210023,China [2]Jiangsu Engineering Research Center of Interfacial Chemistry,School of Chemistry and Chemical Engineering,Nanjing University,Jiangsu,210093,China

出　　处：《Bioactive Materials》2023年第6期376-386,共11页生物活性材料（英文）

基　　金：support from National Natural Science Foundation of China(No.21504046);Natural Science Foundation of Jiangsu Province(No.BK20150970);the Six Talent Peaks Project in Jiangsu Province(SWYY-060);the Projects of Nanjing Normal University(No.184080H20192,184080H10386).

摘　　要：Good’s buffers have been widely applied in cell/organ culture over the past half a century as biocompatible pH stabilizers.However,the emergence of severe adverse effects,such as cellular uptake,lysosomal autophagic activation,and visible light-induced cytotoxicity,raises serious questions over its biocompatibility while underlying mechanism was unclear.Here we report that riboflavin(RF,component of cell culture medium)generates ^(1)O_(2),⋅OH,and O_(2)^(·-)under visible light exposure during regular cell manipulation.These short half-life reactive oxygen species(ROS)react with tertiary amine groups of HEPES,producing 106.6μM of H_(2)O_(2).Orders of magnitude elevated half-life of ROS in the medium caused severe cytotoxicity and systematic disorder of normal cell functions.We have further designed and validated zwitterionic betaines as the new generation biocompatible organic pH buffers,which is able to completely avoid the adverse effects that found on HEPES and derivate Good’s buffers.These findings may also open a new avenue for zwitterionic betaine based materials for biomedical applications.

关 键 词：Good’s buffers Zwitterionic betaines ROS conversion Cellular functions Biocompatibility 

分 类 号：R318[医药卫生—生物医学工程]