Artificial cilia for soft and stable surface covalent immobilization of bone morphogenetic protein-2  

作　　者：Qi Gan Lina Chen Ho-Pan Bei Sze-Wing Ng Han Guo Guoqiang Liu Hao Pan Changsheng Liu Xin Zhao Zijian Zheng 

机构地区：[1]Laboratory for Advanced Interfacial Materials and Devices,School of Fashion and Textiles,The Hong Kong Polytechnic University,Kowloon,99077,Hong Kong Special Administrative Region of China [2]Key Laboratory for Ultrafine Materials of Ministry of Education,School of Materials Science and Engineering,East China University of Science and Technology,Shanghai,200237,PR China [3]Department of Biomedical Engineering,The Hong Kong Polytechnic University,Hung Hom,Hong Kong Special Administrative Region of China [4]Shanghai Synchrotron Radiation Facility,Shanghai Advanced Research Institute,Chinese Academy of Sciences,Shanghai,201204,PR China [5]Department of Applied Biology and Chemical Technology,Faculty of Science,The Hong Kong Polytechnic University,Kowloon,99077,Hong Kong Special Administrative Region of China

出　　处：《Bioactive Materials》2023年第6期551-562,共12页生物活性材料（英文）

基　　金：financial support from RGC Senior Research Fellow Scheme(SRFS2122-5S04),Research Grants Council of Hong Kong;Collaborative Research Fund(C5044-21GF)from the Research Grants Council of Hong Kong and National Excellent Young Scientists Fund(Hong Kong and Macao,82122002),National Natural Science Foundation of China;support from National Natural Science Foundation of China(No.32071337);Shanghai Pujiang Program(20PJ1402600).

摘　　要：Preservation of growth factor sensitivity and bioactivity(e.g.,bone morphogenetic protein-2(BMP-2))post-immobilization to tissue engineering scaffolds remains a great challenge.Here,we develop a stable and soft surface modification strategy to address this issue.BMP-2(a model growth factor)is covalently immobilized onto homogeneous poly(glycidyl methacrylate)(PGMA)polymer brushes which are grafted onto substrate surfaces(Au,quartz glass,silica wafer,or common biomaterials)via surface-initiated atom transfer radical polymerization.This surface modification method multiplies the functionalized interfacial area;it is simple,fast,gentle,and has little effect on the loaded protein owing to the cilia motility.The immobilized BMP-2(i-BMP-2)on the surface of homogeneous PGMA polymer brushes exhibits excellent bioactivity(-87%bioactivity of free BMP-2 in vitro and 20%-50%higher than scaffolds with free BMP-2 in vivo),with conformation and secondary structure well-preserved after covalent immobilization and ethanol sterilization.Moreover,the osteogenic activity of i-BMP-2 on the nanoline pattern(PGMA-poly(N-isopropylacrylamide))shows-110%bioactivity of free BMP-2.This is superior compared to conventional protein covalent immobilization strategies in terms of both bioactivity preservation and therapeutic efficacy.PGMA polymer brushes can be used to modify surfaces of different tissue-engineered scaffolds,which facilitates in situ immobilization of growth factors,and accelerates repair of a wide range of tissue types.

关 键 词：Polymer brush Poly(glycidyl methacrylate) Surface modification Protein immobilization Bioactivity 

分 类 号：TB3[一般工业技术—材料科学与工程]