Novel hybrid biocomposites for tendon grafts:The addition of silk to polydioxanone and poly(lactide-co-caprolactone)enhances material properties,in vitro and in vivo biocompatibility  

作　　者：Behzad Shiroud Heidari Emma Muiños Lopez Emma Harrington Rui Ruan Peilin Chen Seyed Mohammad Davachi Benjamin Allardyce Rangam Rajkhowa Rodney Dilley Froilán Granero-Moltó Elena M.De-Juan-Pardo Minghao Zheng Barry Doyle 

机构地区：[1]Harry Perkins Institute of Medical Research,QEII Medical Centre,Nedlands and the UWA Centre for Medical Research,The University of Western Australia,Perth,Australia [2]School of Engineering,The University of Western Australia,Perth,Australia [3]Australian Research Council Centre for Personalised Therapeutics Technologies,Australia [4]Cell Therapy Area,Centro de Investigaci´on M´edica Aplicada,IDISNA,Universidad de Navarra,Pamplona,Spain [5]Centre for Orthopaedic Research,Faculty of Health and Medical Sciences,The University of Western Australia,Perth,Australia [6]Department of Biology and Chemistry,Texas A&M International University,Laredo,TX,USA [7]Institute for Frontier Materials,Deakin University,Geelong,VIC,3216,Australia [8]Perron Institute for Neurological and Translational Science,Nedlands,Western Australia,Australia

出　　处：《Bioactive Materials》2023年第7期291-306,共16页生物活性材料（英文）

基　　金：The authors also gratefully acknowledge funding from the Australian Research Council(IC170100061)through the Centre for Personalised Therapeutics Technologies,and the Science-Industry PhD Fellowship from the Western Australia Department of Jobs,Tourism,Science and Innovation(awarded to B.S.H.).

摘　　要：Biopolymers play a critical role as scaffolds used in tendon and ligament(TL)regeneration.Although advanced biopolymer materials have been proposed with optimised mechanical properties,biocompatibility,degradation,and processability,it is still challenging to find the right balance between these properties.Here,we aim to develop novel hybrid biocomposites based on poly(p-dioxanone)(PDO),poly(lactide-co-caprolactone)(LCL)and silk to produce high-performance grafts suitable for TL tissue repair.Biocomposites containing 1-15%of silk were studied through a range of characterisation techniques.We then explored biocompatibility through in vitro and in vivo studies using a mouse model.We found that adding up to 5%silk increases the tensile properties,degradation rate and miscibility between PDO and LCL phases without agglomeration of silk inside the composites.Furthermore,addition of silk increases surface roughness and hydrophilicity.In vitro experiments show that the silk improved attachment of tendon-derived stem cells and proliferation over 72 h,while in vivo studies indicate that the silk can reduce the expression of pro-inflammatory cytokines after six weeks of implantation.Finally,we selected a promising biocomposite and created a prototype TL graft based on extruded fibres.We found that the tensile properties of both individual fibres and braided grafts could be suitable for anterior cruciate ligament(ACL)repair applications.

关 键 词：BIOCOMPOSITE Tendon graft SILK Fibre extrusion Biodegradable scaffolds Anterior cruciate ligament 

分 类 号：TB33[一般工业技术—材料科学与工程]