基于网络药理学探讨蒙药翻白草治疗2型糖尿病的作用机制  

Exploring the mechanism of Mongolian medicine of Fanbaicao in treatment of type 2 Diabetes Mellitus based on network pharmacology

作　　者：杭宇昕 于佳琦 曹燕凤 徐宁[1] 刘景林[1] 许良[1] HANG Yuxin;YU Jiaqi;CAO Yanfeng;XU Ning;LIU Jinglin;XU Liang(College of Chemistry and Materials,Inner Mongolia MINZU University,Key Laboratory of Natural Product Chemistry and Functional Molecular Synthesis of Inner Mongolia Autonomous Region,Tongliao 028000,China;Department of Pharmacy of Traditional Chinese Medicine,Beijing University of Chinese Medicine,Beijing 102400,China)

机构地区：[1]内蒙古民族大学化学与材料学院,天然产物化学及功能分子合成内蒙古自治区重点实验室,内蒙古通辽028000 [2]北京中医药大学中药制药系,北京102400

出　　处：《山东第一医科大学（山东省医学科学院）学报》2023年第3期191-196,共6页Journal of Shandong First Medical University ＆ Shandong Academy of Medical Sciences

基　　金：国家自然科学基金(81560702);内蒙古民族大学-蒙古国科学院联合研究课题(NMDGJ0014);内蒙古民族大学博士科研启动基金(BS622);内蒙古自治区本级事业单位引进人才科研启动资金(RCQD202208);内蒙古自然科学基金(2022LH08021);高校科研基本业务费(GXKY22048);内蒙古自治区高校科研一般项目(NJZY23099)。

摘　　要：目的借助网络药理学技术,通过筛选蒙药翻白草活性成分作用靶点和疾病靶点,建立“成分-靶点-疾病”网络,探讨翻白草治疗2型糖尿病的作用机制,为传统蒙药寻找新的作用靶点提供理论依据,为其实验验证及药理研究奠定基础。方法利用中药系统药理数据库分析平台(Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform,TCMSP)查询翻白草活性成分,获得相对应的靶蛋白,通过GeneCards数据库筛选有关2型糖尿病的相关靶点,并利用Venny软件获得翻白草活性成分和糖尿病的靶点交集;使用STRING数据库构建化合物的蛋白互作网络(protein interaction network,PPI),利用Metascape数据库进一步进行基因本体(gene ontology,GO)生物功能以及京都基因和基因组数据库(Kyoto encyclopedia of genes and genomes,KEGG)通路富集分析。结果通过TCMSP数据库查询以及条件筛选,获得5个候选活性分子,潜在靶点共104个,相关通路250条,通过GO和KEGG富集分析得出翻白草主要通过调控癌症通路,糖尿病并发症中AGE-RAGE信号通路以及脂质与动脉粥样硬化相关通路等发挥作用。结论翻白草治疗2型糖尿病并发症主要通过调控AGE-RAGE信号通路,使其具有降低血糖的作用,为进一步实验验证奠定了良好基础。Objective:To explore the mechanism of Mongolian medicine Fanbaicao in treatment of type 2 diabetes by network pharmacology.Methods:All the chemical components related to Fanbaicao were obtained from the TCMSP database by network pharmacology.GeneCard database was searched to obtain the targets of type 2 diabetes,and the intersection targets of active ingredient and disease were obtained by Venny;STRING was used to perform a PPI analysis of the common targets and then construct a PPI network for Fanbaicao in the prevention and treatment of type 2 diabetes.further analysis of target genes of Fanbaicao in GO and KEGG pathway enrichment weremade by using Metascape database.Results:Through TCMSP and condition screening,5 candidate active molecules,104 potential targets and 250 related pathways were obtained.GO and KEGG enrichment analysisconclude showed that Fanbaicao was mainly involved in the pathways in cancer,AGE-RAGE signaling pathway in diabetic complications and lipid and atherosclerosis.Conclusion:The AGE-RAGE signal pathway targeted by Potentilla discolor in the treatment of type 2 diabetes complications has a good effects of regulatory pathway,which has the effect of hypoglycemic,thus laying a good foundation for further experimental verification.

关 键 词：网络药理学 翻白草 2型糖尿病 作用机制 

分 类 号：R29[医药卫生—民族医学]