柿叶黄酮的镇痛抗炎作用及对口腔溃疡大鼠p38MAPK和MMP2/9的调控作用  

Analgesic and anti-inflammatory effects of persimmon leaf flavone and its regulatory effects on p38MAPK and MMP2/9 in rats with oral ulcer

作　　者：王朔 赵作勤 张古泉 张永丽 WANG Shuo;ZHAO Zuoqin;ZHANG Guquan;ZHANG Yongli(School of Stomatology,Shandong First Medical University&Shandong Academy of Medical Science,Taian 27100,China)

机构地区：[1]山东第一医科大学(山东省医学科学院)口腔医学院,山东泰安271016

出　　处：《山东第一医科大学（山东省医学科学院）学报》2023年第3期197-201,共5页Journal of Shandong First Medical University ＆ Shandong Academy of Medical Sciences

基　　金：国家级大学生创新创业训练计划(201510439015);泰安市科技发展计划(2018NS0121)。

摘　　要：目的 探索柿叶黄酮(persimmon leaf flavonoids,PLF)防治口腔溃疡的药效学及其抗炎机制。方法 采用扭体法和痛阈测定考察PLF的镇痛作用;采用耳廓肿胀实验和足趾肿胀实验考察PLF的抗炎作用;通过建立大鼠口腔溃疡模型(醋酸法),采用免疫印迹技术考察PLF对口腔溃疡组织中p38丝裂原活化蛋白激酶(p38 mitogen-activated protein kinases,p38MAPK)、磷酸化p38MAPK(p-p38MAPK)、基质金属蛋白酶-2/9(matrix metalloproteinase-2/9,MMP-2/9)蛋白表达的影响。结果 PLF可显著减少小鼠的扭体次数和提高痛阈值,且PLF可显著减轻二甲苯诱导的小鼠耳廓肿胀度,抑制蛋清所致的足趾肿胀。此外,PLF可显著下调口腔溃疡大鼠溃疡组织内MMP-2/9蛋白表达,显著抑制p38MAPK的磷酸化。结论 PLF具有镇痛抗炎作用,可通过抑制MAPK信号通路及下调MMP-2/9表达发挥防治口腔溃疡的作用。Objective:To explore the pharmacological basis and anti-inflammatory mechanism of persimmon leaf flavonoids(PLF)in preventing and treating oral ulcer.Methods:The analgesic and anti-inflammatory effects of PLF were evaluated by writhing test,pain threshold test,auricle swelling test and toe swelling test in mice.Moreover,based on rat oral uclcer model(acetic acid method),the effects of PLF on the expressions of p38 mitogen activated protein kinases(p38MAPK),phosphorylated p38MAPK(P-P38MAPK)and matrix metalloproteinase-2/9(MMP-2/9)in oral ulcer tissues were investigated by immunoboltting.Results:PLF could significantly reduce the number of writhing,increase the pain threshold,reduce the auricle swelling induced by xylene and inhibit the toe swelling induced by egg white.In addition,PLF could down regulate the expression of MMP-2/9 protein and inhibit the phosphorylation of p38MAPK.Conclusion:PLF has clear analgesic and anti-inflammatory effects.The therapeutic effects of PLF on oral ulcer may be through inhibiting MAPK signaling pathway and down-regulating expression of MMP-2/9.

关 键 词：柿叶黄酮 口腔溃疡 镇痛 炎症 

分 类 号：R285.5[医药卫生—中药学]