Sustained release of hydrogen sulfide from anisotropic ferrofluid hydrogel for the repair of spinal cord injury  

作　　者：Ruofei Wang Xia-Xiao Wu Zhenming Tian Tian Hu Chaoyang Cai Guan-Ping Wu Gang-Biao Jiang Bin Liu 

机构地区：[1]Department of Spine Surgery,The Third Affiliated Hospital of Sun Yat-Sen University,Guangzhou,510630,China [2]Guangdong Provincial Center for Quality Control of Minimally Invasive Spine Surgery,Guangzhou,510630,China [3]Guangdong Provincial Center for Engineering and Technology Research of Minimally Invasive Spine Surgery,Guangzhou,510630,China [4]Key Laboratory for Biobased Materials and Energy of Ministry of Education,College of Materials and Energy,South China Agricultural University,Guangzhou,510642,China

出　　处：《Bioactive Materials》2023年第5期118-128,共11页生物活性材料（英文）

基　　金：This work was supported by the following grants:the National Key Research and Development Program of China(2017YFA0105400);the National Natural Science Foundation of China(82072455,81772349);the Guangdong Basic and Applied Basic Research Foundation(2019A1515012181).The graphical abstract was created with BioR ender.com.

摘　　要：Spinal cord injury(SCI)results in massive neuronal death,axonal disruption,and cascading inflammatory response,which causes further damage to impaired neurons.The survived neurons with damaged function fail to form effective neuronal circuits.It is mainly caused by the neuroinflammatory microenvironment at injury sites and regenerated axons without guidance.To address this challenge,a ferrofluid hydrogel(FFH)was prepared with Ferric tetrasulfide(Fe3S4),carboxymethyl chitosan,and gold.Its internal structural particles can be oriented in a magnetic field to acquire anisotropy.Moreover,Fe3S4 can release hydrogen sulfide(H2S)with anti-inflammatory effects under acidic conditions.Regarding in vitro experiments,0.01g/ml Fe3S4 FFH significantly reduced the inflammatory factors produced by LPS-induced BV2 cells.Oriented and longer axons of the induced neural stem cells loaded on anisotropic FFH were observed.In vivo experiments showed that FFH reduced the activated microglia/macrophage and the expression of pro-inflammatory factors in SCI rats through the NF-κB pathway.Moreover,it significantly promoted directional axonal regrowth and functional recovery after SCI.Given the critical role of inhibition of neuroinflammation and directional axonal growth,anisotropic Fe3S4 FFH is a promising alternative for the treatment of SCI.

关 键 词：Spinal cord injury ANISOTROPIC FERROFLUID Hydrogen sulfide 

分 类 号：R651.2[医药卫生—外科学]