Biodegradable magnesium materials regulate ROS-RNS balance in pro-inflammatory macrophage environment  

作　　者：Maria P.Kwesiga Amani A.Gillette Fatemeh Razaviamri Margaret E.Plank Alexia L.Canull Zachary Alesch Weilue He Bruce P.Lee Roger J.Guillory II 

机构地区：[1]Department of Biomedical Engineering,Michigan Technological University,Houghton,MI,USA [2]Morgridge Institute for Research,Madison,WI,USA [3]Department of Biomedical Engineering,University of Wisconsin-Madison,Madison,WI,USA

出　　处：《Bioactive Materials》2023年第5期261-273,共13页生物活性材料（英文）

基　　金：This work was partially funded by Michigan Tech Research Excellence Fund,National Institutes of Health[grant number R15GM135875(B.P.L.)];the National Science Foundation[grant number DMR 2001076(B.P.L.)],ZA was partially funded by Michigan Tech Undergraduate Research Internship Program.

摘　　要：The relationship between reactive oxygen and nitrogen species(ROS-RNS)secretion and the concomitant biocorrosion of degradable magnesium(Mg)materials is poorly understood.We found that Mg foils implanted short term in vivo(24 h)displayed large amounts of proinflammatory F4/80+/iNOS+macrophages at the interface.We sought to investigate the interplay between biodegrading Mg materials(98.6%Mg,AZ31&AZ61)and macrophages(RAW 264.7)stimulated with lipopolysaccharide(RAW 264.7^(LPS))to induce ROS-RNS secretion.To test how these proinflammatory ROS-RNS secreting cells interact with Mg corrosion in vitro,Mg and AZ61 discs were suspended approximately 2 mm above a monolayer of RAW 264.7 cells,either with or without ^(LPS).The surfaces of both materials showed acute(24 h)changes when incubated in the proinflammatory RAW 264.7^(LPS) environment.Mg discs incubated with RAW 264.7^(LPS) macrophages showed greater corrosion pitting,while AZ61 showed morphological and elemental bulk product changes via scanning electron microscopy-energy dispersive X-ray spectroscopy(SEM-EDX).X-ray photoelectron spectroscopy(XPS)analysis showed a reduction in the Ca/P ratio of the surface products for AZ61 disc incubated with RAW 264.7^(LPS),but not the Mg discs.Moreover,RAW 264.7^(LPS) macrophages were found to be more viable in the acute biodegradative environment generated by Mg materials,as demonstrated by calcein-AM and cleaved(active)caspase-3 staining(CC3).^(LPS) stimulation caused an increase in ROS-RNS,and a decrease in antioxidant peroxidase activity.Mg and AZ61 were found to change this ROS-RNS balance,independently of physiological antioxidant mechanisms.The findings highlight the complexity of the cellular driven acute inflammatory responses to different biodegradable Mg,and how it can potentially affect performance of these materials.

关 键 词：BIOCORROSION Macrophage activation Nitroso-redox balance Mg 

分 类 号：R318.08[医药卫生—生物医学工程]