Transplantation of neural stem progenitor cells from different sources for severe spinal cord injury repair in rat  

作　　者：Bai Xu Man Yin Yaming Yang Yunlong Zou Wenbin Liu Lianyong Qiao Jixiang Zhang Zhan Wang Yayu Wu He Shen Minghan Sun Weiyuan Liu Weiwei Xue Yongheng Fan Qi Zhang Bing Chen Xianming Wu Ya Shi Falong Lu Yannan Zhao Zhifeng Xiao Jianwu Dai 

机构地区：[1]State Key Laboratory of Molecular Developmental Biology,Institute of Genetics and Developmental Biology,Chinese Academy of Sciences,Beijing,100101,China [2]Wuxi School of Medicine,Jiangnan University,Wuxi,214122,China [3]Peking University Health Science Center,Beijing,100191,China [4]China-Japan Union Hospital of Jilin University,Changchun,130033,China [5]Division of Nanobiomedicine,Suzhou Institute of NanoTech and NanoBionics,Chinese Academy of Sciences,Suzhou,215123,China

出　　处：《Bioactive Materials》2023年第5期300-313,共14页生物活性材料（英文）

基　　金：This work was supported by grants from the National Natural Science Foundation of China(No.81891002,No.32071338);the Strategic Priority Research Program of the Chinese Academy of Sciences(XDA16040702,XDA16040704);National Key R&D Program of China(2017YFA0104701,2017YFA0104704);CAS Project for Young Scientists in Basic Research(YSRB073).

摘　　要：Neural stem progenitor cell(NSPC)transplantation has been regarded as a promising therapeutic method for spinal cord injury(SCI)repair.However,different NSPCs may have different therapeutic effects,and it is therefore important to identify the optimal NSPC type.In our study,we compared the transcriptomes of human fetal brain-derived NSPCs(BNSPCs),spinal cord-derived NSPCs(SCNSPCs)and H9 embryonic stem-cell derived NSPCs(H9-NSPCs)in vitro and subsequently we transplanted each NSPC type on a collagen scaffold into a T8-9 complete SCI rat model in vivo.In vitro data showed that SCNSPCs had more highly expressed genes involved in nerve-related functions than the other two cell types.In vivo,compared with BNSPCs and H9-NSPCs,SCNSPCs exhibited the best therapeutic effects;in fact,SCNSPCs facilitated electrophysiological and hindlimb functional recovery.This study demonstrates that SCNSPCs may be an appropriate candidate cell type for SCI repair,which is of great clinical significance.

关 键 词：Spinal cord injury Brain-derived NSPCs Spinal cord-derived NSPCs H9 embryonic stem cell-derived NSPCs Collagen scaffolds 

分 类 号：R651.2[医药卫生—外科学]