Constructing a highly bioactive tendon-regenerative scaffold by surface modification of tissue-specific stem cell-derived extracellular matrix  

作　　者：Liang-Ju Ning Jing Cui Shu-Kun He Ruo-Nan Hu Xuan Yao Yi Zhang Wei Ding Yan-Jing Zhang Jing-Cong Luo Ting-Wu Qin 

机构地区：[1]Laboratory of Stem Cell and Tissue Engineering,Orthopedic Research Institute,State Key Laboratory of Biotherapy and Cancer Center,West China Hospital,Sichuan University and Collaborative Innovation Center of Biotherapy,Chengdu,Sichuan 610041,P.R.China [2]Department of Orthopedic Surgery,Orthopedic Research Institute,West China Hospital,Sichuan University,Chengdu,Sichuan 610041,P.R.China [3]Core Facility,West China Hospital,Sichuan University,Chengdu,Sichuan 610041,P.R.China

出　　处：《Regenerative Biomaterials》2022年第1期81-92,共12页再生生物材料（英文版）

基　　金：supported by the grants from National Natural Science Foundation of China(grant numbers:32171349,31600783,31870968);Science and Technology Plan of Sichuan Province(grant number:2018SZ0044).

摘　　要：Developing highly bioactive scaffold materials to promote stem cell migration,proliferation and tissue-specific differentiation is a crucial requirement in current tissue engineering and regenerative medicine.Our previous work has demonstrated that the decellularized tendon slices(DTSs)are able to promote stem cell proliferation and tenogenic differentiation in vitro and show certain pro-regenerative capacity for rotator cuff tendon regeneration in vivo.In this study,we present a strategy to further improve the bioactivity of the DTSs for constructing a novel highly bioactive tendon-regenerative scaffold by surface modification of tendon-specific stem cell-derived extracellular matrix(tECM),which is expected to greatly enhance the capacity of scaffold material in regulating stem cell behavior,including migration,proliferation and tenogenic differentiation.We prove that the modification of tECM could change the highly aligned surface topographical cues of the DTSs,retain the surface stiffness of the DTSs and significantly increase the content of multiple ECM components in the tECM-DTSs.As a result,the tECM-DTSs dramatically enhance the migration,proliferation as well as tenogenic differentiation of rat bone marrow-derived stem cells compared with the DTSs.Collectively,this strategy would provide a new way for constructing ECMbased biomaterials with enhanced bioactivity for in situ tendon regeneration applications.

关 键 词：tendon-regenerative scaffold extracellular matrix bioactive material tissue-specific stem cell 

分 类 号：R318[医药卫生—生物医学工程]