Three-dimensional-printed polycaprolactone scaffolds with interconnected hollow-pipe structures for enhanced bone regeneration  

作　　者：Jiahua Duan Dong Lei Chen Ling Yufeng Wang Zhicheng Cao Ming Zhang Huikang Zhang Zhengwei You Qingqiang Yao 

机构地区：[1]Department of Orthopaedic Surgery,Nanjing First Hospital,Nanjing Medical University,Nanjing 210006,China [2]State Key Laboratory for Modification of Chemical Fibers and Polymer Materials,College of Materials Science and Engineering,Institute of Functional Materials,Donghua University,Shanghai 201620,China [3]Research Base of Textile Materials for Flexible Electronics and Biomedical Applications(China Textile Engineering Society),Shanghai Engineering Research Center of Nano-Biomaterials and Regenerative Medicine,Shanghai 201620,China [4]Department of Cardiology,Shanghai 9th People’s Hospital,Shanghai Key Laboratory of Tissue Engineering,School of Medicine,Shanghai Jiao Tong University,Shanghai 200011,China

出　　处：《Regenerative Biomaterials》2022年第1期471-479,共9页再生生物材料（英文版）

基　　金：supported by the National Natural Science Foundation of China(82072400,82102211,52173117);the Natural Science Foundation of Jiangsu Province(BK20200001);the Natural Science Foundation of Shanghai(20ZR1402500);the Belt&Road Young Scientist Exchanges Project of Science and Technology Commission Foundation of Shanghai(20520741000);Ningbo 2025 Science and Technology Major Project(2019B10068);the Science and Technology Commission of Shanghai Municipality(20DZ2254900,20DZ2270800);the Fundamental Research Funds for the Central Universities,DHU Distinguished Young Professor Program(LZA2019001).

摘　　要：Three-dimensional(3D)-printed scaffolds are widely used in tissue engineering to help regenerate critical-sized bone defects.However,conventional scaffolds possess relatively simple porous structures that limit the delivery of oxygen and nutrients to cells,leading to insufficient bone regeneration.Accordingly,in the present study,perfusable and permeable polycaprolactone scaffolds with highly interconnected hollow-pipe structures that mimic natural micro-vascular networks are prepared by an indirect onepot 3D-printing method.In vitro experiments demonstrate that hollow-pipe-structured(HPS)scaffolds promote cell attachment,proliferation,osteogenesis and angiogenesis compared to the normal non-hollow-pipe-structured scaffolds.Furthermore,in vivo studies reveal that HPS scaffolds enhance bone regeneration and vascularization in rabbit bone defects,as observed at 8 and 12weeks,respectively.Thus,the fabricated HPS scaffolds are promising candidates for the repair of critical-sized bone defects.

关 键 词：three-dimensional printing interconnected hollow-pipe structure bone regeneration VASCULARIZATION POLYCAPROLACTONE 

分 类 号：R318[医药卫生—生物医学工程]