In vitro and in vivo studies on bacteria and encrustation resistance of heparin/poly-L-lysine-Cu nanoparticles coating mediated by PDA for ureteral stent application  

作　　者：Bukola O.Awonusi Jianzhong Li Hongwei Li Zhenyu Wang Ke Yang Jing Zhao 

机构地区：[1]Shi-changxu Innovation Center for Advanced Materials,Institute of Metal Research,Chinese Academy of Sciences,Shenyang 110016,China [2]School of Materials Science and Engineering,University of Science and Technology of China,Hefei,230026,China [3]Department of Urology,General Hospital of Northern Theater Command,Shenyang,110840,China

出　　处：《Regenerative Biomaterials》2022年第1期625-635,共11页再生生物材料（英文版）

基　　金：Liaoning Science and Technology Program(grant No.2020JH2/10300159).

摘　　要：Ureteral stents are commonly utilized as a medical device to aid the flow of urine.However,biofilm formation and encrustation complications have been clinical problems.To overcome this challenge,heparin/poly-L-lysine-copper(Hep/PLL-Cu)nanoparticle was immobilized on a dopamine-coated polyurethane surface(PU/NPs).The stability and structural properties of the nanoparticles were characterized by Zeta potential,poly dispersion index,transmission electron microscopy,atom force microscopy and contact angle.The surface composition,antibacterial potency,encrustation resistance rate and biocompatibility of PU/NPs were investigated by scanning electron microscope,X-ray photoelectron spectroscopy,antibacterial assay and MTS assay,respectively.In addition,the antiencrustation property was studied by implanting coated NPs stents in the rat bladder for 7 days.It was shown that the size and distribution of Hep/PLL-Cu nanoparticles were uniform.PU/NPs could inhibit Proteus mirabilis proliferation and biofilm formation,and exhibit no cytotoxicity.Less encrustation(Ca and Mg salt)was deposited both in vitro and in vivo on samples,demonstrating that the NPs coating could be a potential surface modification method of ureteral material for clinical use.

关 键 词：ureteral stents nanoparticles BIOFILM ENCRUSTATION 

分 类 号：TB383[一般工业技术—材料科学与工程]