Frozen bean curd-inspired xenogeneic acellular dermal matrix with triple pretreatment approach of freeze–thaw,laser drilling and ADSCs pre-culture for promoting early vascularization and integration  

作　　者：Xing Huang Zhu Zhu Lin Lu Rui Jin Di Sun Xusong Luo 

机构地区：[1]Department of Plastic and Reconstructive Surgery,Shanghai 9th People’s Hospital,Shanghai Jiao Tong University School of Medicine,Shanghai 200011,PR China [2]Shanghai Key Lab of Tissue Engineering,Shanghai 9th People’s Hospital,Shanghai Jiao Tong University School of Medicine,Shanghai 200011,PR China

出　　处：《Regenerative Biomaterials》2022年第1期687-703,共17页再生生物材料（英文版）

基　　金：supported by the Shanghai Committee of Science and Technology,China(grant no.19ZR1430100);National Natural Science Foundation of China(grant no.81371701);the Shanghai Municipal Key Clinical Specialty,China(grant no.shslczdzk00901).

摘　　要：Xenogeneic acellular dermal matrix(ADM)is widely used in clinical practice given its good biocompatibility and biomechanical properties.Yet,its dense structure remains a hindrance.Incorporation of laser drilling and pre-culture with Adipose-derived stem cells(ADSCs)have been attempted to promote early vascularization and integration,but the results were not ideal.Inspired by the manufacturing procedure of frozen bean curd,we proposed a freeze–thaw treatment to enhance the porosity of ADM.We found that the ADM treated with-80℃3Rt-30℃3R had the largest disorder of stratified plane arrangement(deviation angle 28.6%)and the largest porosity(96%),making it an optimal approach.Human umbilical vein endothelial cells on freeze–thaw treated ADM demonstrated increased expression in Tie-2 and CD105 genes,proliferation,and tube formation in vitro compared with those on ADM.Combining freeze–thaw with laser drilling and pre-culture with ADSCs,such tri-treatment improved the gene expression of proangiogenic factors including IGF-1,EGF and vascular endothelial growth factor,promoted tube formation,increased cell infiltration and accelerated vascularization soon after implantation.Overall,freeze–thaw is an effective method for optimizing the internal structure of ADM,and tri-treatments may yield clinical significance by promoting early cell infiltration,vascularization and integration with surrounding tissues.

关 键 词：FREEZE-THAW xenogeneic acellular dermal matrix VASCULARIZATION early integration 

分 类 号：TN24[电子电信—物理电子学]