Addition of collagen type I in agarose created a dose-dependent effect on matrix production in engineered cartilage  

作　　者：Gabriel R.López-Marcial Keerthana Elango Grace D.O’Connell 

机构地区：[1]Department of Mechanical Engineering,University of California-Berkeley,6141 Etcheverry Hall,Berkeley CA 94720,USA [2]Department of Orthopaedic Surgery,University of California-San Francisco,500 Parnassus Ave MU-320W,San Francisco CA 94143,USA

出　　处：《Regenerative Biomaterials》2022年第1期704-709,共6页再生生物材料（英文版）

基　　金：supported by the National Science Foundation(NSF),Grant Number[DGE 1752814].

摘　　要：Extracellular-matrix composition impacts mechanical performance in native and engineered tissues.Previous studies showed collagen typeⅠ-agarose blends increased cell-matrix interactions and extracellular matrix production.However,long-term impacts on protein production and mechanical properties of engineered cartilage are unknown.Our objective was to characterize the effect of collagen type I on the matrix production of chondrocytes embedded in agarose hydrogels.We hypothesized that the addition of collagen would improve longterm mechanical properties and matrix production(e.g.collagen and glycosaminoglycans)through increased bioactivity.Agarose hydrogels(2%w/v)were mixed with varying concentrations of collagen typeⅠ(0,2 and 5 mg/ml).Juvenile bovine chondrocytes were added to the hydrogels to assess matrix production over 4 weeks through biochemical assays,and mechanical properties were assessed through unconfined compression.We observed a dose-dependent effect on cell bioactivity,where 2 mg/ml of collagen improved bioactivity,but 5 mg/ml had a negative impact on bioactivity.This resulted in a higher modulus for scaffolds supplemented with lower collagen concentration as compared to the higher collagen concentration,but not when compared to the control.In conclusion,the addition of collagen to agarose constructs provided a dose-dependent impact on improving glycosaminoglycan production but did not improve collagen production or compressive mechanics.

关 键 词：HYDROGEL tissue engineering composition agarose-collagen matrix MECHANICS 

分 类 号：R318[医药卫生—生物医学工程]