Laminin 332-functionalized coating to regulate the behavior of keratinocytes and gingival mesenchymal stem cells to enhance implant soft tissue sealing  

作　　者：Lipeng Liu Jing Wang Ying Li Bing Liu Wei Zhang Weikang An Qing Wang Boya Xu Lingzhou Zhao Chufan Ma 

机构地区：[1]State Key Laboratory of Military Stomatology&National Clinical Research Center for Oral Diseases&Shaanxi Key Laboratory of Stomatology,Department of Prosthodontics,School of Stomatology,The Fourth Military Medical University,Xi’an,Shaanxi 710032,China [2]Department of Stomatology,Air Force Medical Center,The Fourth Military Medical University,Beijing 100142,China

出　　处：《Regenerative Biomaterials》2022年第1期724-738,共15页再生生物材料（英文版）

基　　金：supported by the National Natural Science Foundation of China(No.81970971);Shaanxi Key Research and Development Program(No.2022SF-179).

摘　　要：Peri-implant epithelial sealing is the first line of defense against external pathogens or stimuli;hence,an essential process to prevent peri-implantitis.Laminin 332(LN332)is the main component of the internal basal lamina and participates in peri-implant epithelial sealing by forming hemidesmosomes(HDs)with integrinα6β4.In thiswork,poly(D,L-lactide)(PDLLA)-LN332 composite coating was successfully constructed by a method similar to layer-by-layer assembly,displaying staged LN332 release for as long as 28days.The PDLLA-LN332 composite coating can activate the intracellular PI3K-Akt pathway via binding to cellular integrinα6β4,which can promote adhesion,migration and proliferation of HaCaT cells and further enhance the expression of keratinocyte HD-related molecules,including integrinα6β4,LN332 and plectin.Furthermore,the PDLLA-LN332 composite coating can promote the adhesion,spreading and proliferation of gingival mesenchymal stem cells and accelerate their epithelial differentiation.Therefore,the PDLLA-LN332 composite coating can enhance implant soft tissue sealing,warranting further in vivo study.

关 键 词：PDLLA laminin 332 KERATINOCYTES gingival mesenchymal stem cells soft tissue sealing 

分 类 号：R783.1[医药卫生—口腔医学]