Promoting effect of a calcium-responsive self-assemblyβ-sheet peptide on collagen intrafibrillar mineralization  

作　　者：Zhongcheng Li Qian Ren Sili Han Longjiang Ding Xi Qin Die Hu Ting He Tian Tian Ziqian Lu Linglin Zhang 

机构地区：[1]State Key Laboratory of Oral Diseases,National Clinical Research Centre for Oral Diseases,Department of Cariology and Endodontics,West China Hospital of Stomatology,Sichuan University,Chengdu 610041,Sichuan,China [2]Department of Oral Medicine,Shenzhen Stomatology Hospital,Shenzhen 518038,Guangdong,China

出　　处：《Regenerative Biomaterials》2022年第1期761-774,共14页再生生物材料（英文版）

基　　金：supported by the National Natural Science Foundation of China(Grant Nos.81470734,Grant Nos.81970931).

摘　　要：Recently,a de novo synthetic calcium-responsive self-assemblyβ-sheet peptide ID8(Ile-Asp-Ile-Asp-Ile-Asp-Ile-Asp)has been developed to serve as the template inducing hydroxyapatite nucleation.The aim of this study was to evaluate the effect of ID8 on intrafibrillar mineralization of collagen making full use of its self-assembly ability.The mineralization experiments were carried out in vitro on both bare TypeⅠcollagen and fully demineralized dentin samples.The calcium-responsive self-assembly of ID8 was revealed by circular dichroism spectrum,8-anilino-1-naphthalenesulfonic acid ammonium salt hydrate assay,attenuated total reflection Fourier transform infrared spectrum(ATR-FTIR)and transmission electron microscope(TEM).Polyacrylic acid(450 kDa)with a concentration of 100μg ml^(-1)was selected as the nucleation inhibitor based on the determination of turbidimetry and TEM with selected area electron diffraction(TEM-SAED).The results showed that collagen intrafibrillar mineralization was significantly promoted with the pretreatment of self-assembly ID8 detected by TEM-SAED,SEM,X-ray diffraction and ATRFTIR.The pretreatment of collagen utilizing self-assembly ID8 not only enhanced intermolecular hydrogen bonding but also contributed to calcium retention inside collagen and significantly increased the hydrophilicity of collagen.These results indicated that peptides with self-assembly properties like ID8 are expected to be potential tools for biomimetic mineralization of collagen.

关 键 词：COLLAGEN polyacrylic acid BIOMINERALIZATION biomimetic materials amorphous calcium phosphate 

分 类 号：O63[理学—高分子化学]