In vivo study on heparin/poly-L-lysine-copper coating for surface functionalization of ureteral stent  

作　　者：Bukola O.Awonusi Jianzhong Li Hongwei Li Zhenyu Wang Jiyuan Hu Ke Yang Jing Zhao Shi-Changxu 

机构地区：[1]Innovation Center for Advanced Materials,Institute of Metal Research,Chinese Academy of Sciences,Shenyang 110016,China [2]School of Materials Science and Engineering,University of Science and Technology of China,Hefei 230026,China [3]Department of Urology,General Hospital of Northern Theater Command,Shenyang 110840,China [4]Department of Urology,The First Affiliated Hospital of China Medical University,Shenyang 110001,China

出　　处：《Regenerative Biomaterials》2022年第1期955-962,共8页再生生物材料（英文版）

基　　金：Liaoning Science and Technology Program(grant No.2020JH2/10300159).

摘　　要：Polyurethane(PU)ureteral stents are used in clinics to maintain the ureteral patency.Due to biofilm formation and encrustation complications,long-term clinical usage has been limited.It is therefore necessary to develop an effective response to this unmet medical need.A heparin/poly-L-lysine/copper(NPs)coating was developed in our previous work that showed the effect of preventing infection and encrustation in vitro.In this work,a further study was conducted by grafting NPs on clinical ureteral stents that then were implanted into the infectious bladders of Wistar rats to investigate the effects of nanoparticles on bacterial growth and crystal deposition in vivo.It was found that decreased numbers of adherent microbes,urease amount splitting by bacteria,and deposited crystals were observed on the NPs stents with significant differences in comparison with PU stents.Besides,histological analysis showed that the NPs stents decreased the host tissue inflammation in close relation to the decrease biofilm formation and encrustation after 28 days of implantation.

关 键 词：ureteral stent heparin/PLL-Cu nanoparticles in vivo implantation bacterial adhesion ENCRUSTATION 

分 类 号：R318[医药卫生—生物医学工程]