甘蓝型油菜显性核不育系8029AB SNP标记开发与应用  

Development of the SNP marker of the dominant genic male sterility line 8029AB and its utilization in rapeseed(Brassica napus L.)breeding

作　　者：李真[1] 袁荣 王淼 朱莉[2] 李晓飞[2] 洪美艳 郭瑞星[2] 曾新华[2] LI Zhen;YUAN Rong;WANG Miao;ZHU Li;LI Xiao-fei;HONG Mei-yan;GUO Rui-xing;ZENG Xin-hua(School of Agriculture,Jinhua Polytechnic,Jinhua 321007,China;Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences/Key Laboratory of Biology and Genetic Improvement of Oil Crops,Wuhan 430062,China)

机构地区：[1]金华职业技术学院农学院,浙江金华321007 [2]中国农业科学院油料作物研究所/农业部油料作物生物学与遗传育种重点实验室,湖北武汉430062

出　　处：《中国油料作物学报》2023年第2期253-259,共7页Chinese Journal of Oil Crop Sciences

基　　金：浙江省自然科学基金(LQ18C060004);金华职业技术学院国家级培育项目(PY202105)。

摘　　要：8029AB是新选育的甘蓝型油菜细胞核显性雄性不育(DGMS)两型系,其育性稳定,受1对复等位基因(BnMS5^(a)/BnMS5^(c)/BnMS5^(e))控制,可用于油菜杂交种的三系制种。为推进8029AB在育种中的应用,需开发功能标记以准确地鉴定复等位基因(BnMS5^(a)/BnMS5^(c)/BnMS5^(e))。基于此,本研究分别根据BnMS5^(a)、BnMS5^(c)和BnMS5^(e)的基因序列设计了15对SNP标记,并对其进行筛选及验证。结果表明:SNP标记SNPMS5^(a)-5与BnMS5^(a)基因紧密连锁,可在BnMS5^(a)可育个体中扩增200 bp的条带;SNP标记SNPMS5^(c)-3与BnMS5^(c)基因紧密连锁,可以扩增出140 bp的条带;与BnMS5^(e)紧密连锁的SNP标记SNPMS5^(e)-1在含有BnMS5^(e)的植株上扩增出150 bp的条带。经过验证,这些SNP标记可以精确判断BnMS5^(a)/BnMS5^(c)/BnMS5^(e)是否存在。因此,可以在前期利用这些SNP标记进行分子标记辅助选择(MAS),缩短育种周期,提高育种效率,实现甘蓝型油菜DGMS三系杂交制种的规模化及商业化生产。8029AB is a newly bred dominant genic male sterility(DGMS)two-type line in Brassica napus,whose fertility is controlled by one gene with three different alleles(BnMS5^(a)/BnMS5^(c)/BnMS5^(e)).It can be used in a three-line approach for the seed production of rapeseed hybrids.In order to promote its application,functional markers should be developed to accurately identify multiple alleles(BnMS5^(a)/BnMS5^(c)/BnMS5^(e)).In our study,15 pairs of SNP markers were designed according to the sequence of BnMS5^(a),BnMS5^(c) and BnMS5^(e).Three of them were successfully identified and validated.The SNP marker SNPMS5^(a)-5 was tightly linked to BnMS5^(a),which could am⁃plify a 200 bp band in fertile individuals with BnMS5^(a).SNPMS5^(c)-3 could be used as the molecular marker of BnMS5^(c) through amplifying a 140 bp band.SNPMS5^(e)-1 which was tightly linked to BnMS5^(e) could amplify one band of 150 bp in plants with BnMS5^(e).These SNP markers could predict the presence of BnMS5^(a)/BnMS5^(c)/BnMS5^(e) effec⁃tively.Consequently,marker-assisted selection can be carried out in the early generation to shorten the breeding pe⁃riod and improve the breeding efficiency,so as to realize large-scale commercial production of DGMS three-line hy⁃brid seed in rapeseed.

关 键 词：甘蓝型油菜 显性核不育 SNP 分子标记辅助选择 

分 类 号：S565.4[农业科学—作物学]