大豆株高QTL定位及候选基因挖掘  

QTL mapping and candidate gene mining for soybean plant height

作　　者：郑海洋 侯立龙 曹富斌 魏茹茹 谢建国 蒋洪蔚 陈庆山[1] ZHENG Hai-yang;HOU Li-long;CAO Fu-bin;WEI Ru-ru;XIE Jian-guo;JIANG Hong-wei;CHEN Qing-shan(Northeast Agricultural University/Ministry of Education Soybean Biology Key Laboratory/Soybean Genetic Improvement Team,Harbin 150030,China;Jilin Academy of Agricultural Sciences/Soybean Research Institute,Changchun 130033,China)

机构地区：[1]东北农业大学/教育部大豆生物学重点实验室/大豆遗传改良团队,黑龙江哈尔滨150030 [2]吉林省农业科学院/大豆研究所,吉林长春130033

出　　处：《中国油料作物学报》2023年第2期283-292,共10页Chinese Journal of Oil Crop Sciences

基　　金：国家自然科学基金(U20A2027,31771882);财政部和农业农村部:国家现代农业产业技术体系(CARS-04-PS07,CARS-04-PS11);吉林省科研院所引进高层次科研创新人才资助计划(0413502)。

摘　　要：大豆株高(plant height,PH)对理想株型和产量有重要影响,为筛选株高相关候选基因,以包含208个株系的染色体片段代换系(chromosome segment substitution lines,CSSL)群体为材料,对大豆株高性状进行QTL定位。共检测到12个与大豆株高相关的QTL位点,其中qPH7-1、qPH12-1和qPH16-1在不同年份被重复检测到。对3个QTL区间进行基因注释得到35个基因。根据基因注释、双亲序列比对和qRT-PCR,最终预测Glyma.16G198800和Glyma.16G199000为调控大豆株高的候选基因。单倍型分析结果显示Glyma.16G198800中SNP-4034在双亲间的差异可能是导致株高产生差异的原因。For high yield soybean plant type breeding,QTL mapping for soybean plant height was performed by CSSLs(chromosome segment substitution lines).Among 208 lines in the CSSL population,12 QTLs associated with plant height were detected.Among the QTLs,qPH7-1,qPH12-1 and qPH16-1 could be repeatedly detected in two years,which containing 35 genes.According to gene annotation,biparental sequence alignment and qRTPCR,Glyma.16G198800 and Glyma.16G199000 were predicted as candidate genes regulating soybean plant height.Haplotype analysis results showed that the difference of SNP-4034 in Glyma.16G198800 between two parents might be responsible for plant height difference.The above results were expected to provide theoretical basis for soy⁃bean plant type breeding.

关 键 词：大豆 染色体片段代换系(CSSL) 株高 QTL 候选基因 

分 类 号：S565.1[农业科学—作物学]