基于共线性分析的大豆种子硬实性QTL定位及候选基因预测  

QTL mapping and prediction of candidate gene for seed hardness based on collinearity analysis

作　　者：马迅通 吉彪 马占洲 董晓慧[4] 韩思宁 韩留阳 李旭[1] 刘佳瑞 张钰 冯学珍 张煦杭 黄亚娸 陈庆山[1] 齐照明[1] 武小霞[1] MA Xun-tong;JI-Biao;MA Zhan-zhou;DONG Xiao-hui;HAN Si-ning;HAN Liu-yang;LI-Xu;LIU Jia-rui;ZHANG-Yu;FENG Xue-zhen;ZHANG Xu-hang;HUANG Ya-qi;CHEN Qing-shan;QI Zhao-ming;WU Xiao-xia(Northeast Agricultural University,Harbin 150030,China;Jiangsu Vocational College of Agriculture and Forestry,Zhenjiang 212000,China;Nenjiang Agricultural Technology Extension Center,Heihe 161400,China;Suihua Branch of Heilongjiang Academy of Agricultural Sciences,Suihua 152000,China;Sinochim Agriculture Holdings,Shanghai 200120,China)

机构地区：[1]东北农业大学,黑龙江哈尔滨150030 [2]江苏农林职业技术学院,江苏镇江212000 [3]嫩江市农业技术推广中心,黑龙江黑河市161400 [4]黑龙江省农业科学院绥化分院,黑龙江绥化152000 [5]中化现代农业有限公司,上海200120

出　　处：《中国油料作物学报》2023年第2期293-300,共8页Chinese Journal of Oil Crop Sciences

基　　金：先正达集团东北农业大学联合基金(86011700)。

摘　　要：大豆种子硬实度关乎食品与饲料加工。为提高大豆种子硬实度分子标记辅助选择的效率,发现相关候选基因,利用染色体片段代换系(chromosome segment substitution lines,CSSL)对大豆种子硬实性进行了两年的QTL定位研究,结合前人得到的25个种子硬实性QTL,利用MCScanX对整个大豆基因组进行分析,生成共线性区组,评估大豆种子硬实性QTL之间的共线性,确定了位于Gm02片段的中心QTL。利用多个数据库分析hub-QTL区段的基因,锚定了8个与大豆种子硬实性相关的候选基因。从CSSL群体中选择种子硬实性不同的两个品系和轮回亲本,用于随后的实时荧光定量PCR分析,发现候选基因Glyma.02G269400和Glyma.02G269500在CSSL群体中硬实性不同的2个品系及轮回亲本绥农14中的表达差异显著。Glyma.02G262600在绥农14中的相对表达量约是CSSL-136的5倍,而在CSSL-200中表达量中等,推测该基因抑制大豆种皮的形成。Soybean seed hardness is related to food and feed processing.For molecular marker-assisted selec⁃tion of soybean seed hardness,we found related candidate genes,and used chromosome segment substitution lines(CSSL)to localize QTL for soybean seed hardness for two years.Combined with the 25 seed hardness QTL obtained previously,MCScanX was used to analyze the whole soybean genome to generate collinearity block,evaluate the col⁃linearity among the seed hardness QTL,and identify the central QTL located in Gm02 fragment.Multiple databases were used to analyze the genes of the Hub-QTL region and anchor.Eight candidate genes related to seed hardness in soybean.Two lines with different seed hardness and recurrent parents were selected from the CSSL population for subsequent quantitative real-time PCR analysis.It was found that the expression of candidate genes Gly⁃ma.02G269400 and Glyma.02G269500 were significantly different in the two strains of CSSL population and the re⁃current parent Suinong 14.The relative expression level of Glyma.02G262600 in Suisunong 14 was about 5 times that of CSSL-136,while the expression level of Glyma.02G262600 in CSSL-200 was moderate,suggesting that this gene inhibited the formation of soybean seed coat.

关 键 词：大豆 种子硬实 QTL定位 共线性分析 

分 类 号：S565.1[农业科学—作物学]