花生籽仁大小相关性状QTL定位分析  

QTL mapping for seed size in cultivated peanut(Arachis hypogaea L.)

作　　者：郭朋霞 姜慧芳[1] 游宇 李威涛 喻博伦 罗怀勇[1] 黄莉[1] 刘念[1] 陈伟刚[1] 雷永[1] 廖伯寿[1] 周小静[1] GUO Peng-xia;JIANG Hui-fang;YOU Yu;LI Wei-tao;YU Bo-lun;LUO Huai-yong;HUANG Li;LIU Nian;CHEN Wei-gang;LEI Yong;LIAO Bo-shou;ZHOU Xiao-jing(Oil Crops Research Institute,Chinese Academy of Agricultural Sciences/Key Laboratory of Biology and Genetic Improvement of Oil Crops,Ministry of Agriculture and Rural Affairs,Wuhan 430062,China;Nanchong Academy of Agricultural Sciences,Nanchong 637000,China)

机构地区：[1]中国农业科学院油料作物研究所/农业农村部油料作物生物学与遗传育种重点实验室,湖北武汉430062 [2]四川省南充市农业科学研究所,四川南充637000

出　　处：《中国油料作物学报》2023年第2期301-308,共8页Chinese Journal of Oil Crop Sciences

基　　金：国家自然科学基金(32172066);农作物种质资源保护项目(19210163);国家花生产业技术体系(CARS-13);花生种质资源共享服务平台项目(NCGRC-2021-016)。

摘　　要：籽仁是衡量花生产量的重要指标,为研究花生籽仁大小的遗传基础,本研究以中花5号和ICGV 86699及其杂交衍生的RIL群体为材料,对籽仁长和籽仁宽在五个环境、百仁重在六个环境进行性状考察及QTL定位分析。结果表明,花生籽仁长、籽仁宽和百仁重在RIL群体表现出连续变异,呈正态分布,且性状间呈极显著正相关。在多环境下共定位到与籽仁长、籽仁宽和百仁重相关的QTL分别为7、10和15个,在鉴定到的QTL中有9个表型贡献率大于10%,位于A05染色体上的籽仁宽QTL qSWA05.1贡献率最大(33.22%)。位于B06染色体上与籽仁长相关的稳定主效QTL qSLB06.1和qSLB06.2在四个环境中均被重复检测到,分别解释9.02%~21.53%和12.54%~26.55%的表型变异,与百仁重相关的稳定主效QTL qHSWB06.1和qHSWB06.2分别在3个和6个环境中重复检测到,表型贡献率为5.89%~14.75%和6.09%~17.89%。在B04、B06和B10染色体上存在3、2和1个籽仁大小不同性状的共定位区间。本研究为花生产量性状的精细定位和遗传改良奠定理论基础。Seed size is important to peanut yield.In order to study the genetic basis of seed size,trait in⁃vestigation and QTL mapping of seed length and seed width in 5 environments and hundred-seed weight in 6 environments were performed using a recombinant inbred lines(RIL)population derived from Zhonghua 5×ICGV 86699.The results showed that seed size-related traits showed continuous variation and normal distribu⁃tion in RIL population,and there was a very significant positive correlation among traits.A total of 7,10 and 15 QTLs related to seed length,seed width and hundred-seed weight were identified in multi-environments,re⁃spectively.There were 9 QTLs with the explained phenotypic variance(PVE)more than 10%,and the QTL qSWA05.1 for seed width had the largest PVE(33.22%).The stable major QTL qSLB06.1 and qSLB06.2 for seed length were repeatedly detected in 4 environments,explaining the phenotypic variation of 9.02%-21.53%and 12.54%-26.55%respectively.The stable major QTL qHSWB06.1 and HSWB06.2 for hundred-seed weight were repeatedly detected in 3 and 6 environments,explaining the phenotypic variation of 5.89%-14.75%and 6.09%-17.89%respectively.There were 3,2 and 1 colocation intervals for seed size-related traits on chromo⁃somes B04,B06 and B10,respectively.This study laid a theoretical foundation for fine mapping and genetic improvement of yield traits in peanut.

关 键 词：花生 籽仁大小 QTL定位 共定位 

分 类 号：S565.2[农业科学—作物学]