Seamless and early gap healing of osteochondral defects by autologous mosaicplasty combined with bioactive supramolecular nanofiber-enabled gelatin methacryloyl(BSN-GelMA)hydrogel  

作　　者：Hongwei Wu Yuna Shang Wei Sun Xinyi Ouyang Wenyan Zhou Jieji Lu Shuhui Yang Wei Wei Xudong Yao g Xiaozhao Wang Xianzhu Zhang Yishan Chen Qiulin He Zhimou Yang Hongwei Ouyang 

机构地区：[1]Dr.Li Dak Sum&Yip Yio Chin Center for Stem Cells and Regenerative Medicine,and Department of Orthopedic Surgery of the Second Affiliated Hospital,Zhejiang University School of Medicine,Hangzhou,China [2]Department of Sports Medicine,Zhejiang University School of Medicine,Hangzhou,China [3]Zhejiang University-University of Edinburgh Institute,Zhejiang University School of Medicine,and Key Laboratory of Tissue Engineering and Regenerative Medicine of Zhejiang Province,Zhejiang University School of Medicine,Hangzhou,China [4]China Orthopedic Regenerative Medicine Group(CORMed),Hangzhou,China [5]College of Chemistry,Tianjin Normal University,Tianjin,300387,PR China [6]Faculty of Science,The University of Melbourne,Parkville,VIC,3010,Australia [7]International Institutes of Medicine,The 4th Affiliated Hospital of Zhejiang University School of Medicine,Yiwu,Zhejiang,China [8]Key Laboratory of Bioactive Materials,Ministry of Education,State Key Laboratory of Medicinal Chemical Biology,College of Life Sciences,Collaborative Innovation Center of Chemical Science and Engineering,and National Institute of Functional Materials,Nankai University,Tianjin,300071,China

出　　处：《Bioactive Materials》2023年第1期88-102,共15页生物活性材料（英文）

基　　金：supported by the National Key Research and Development Program of China(2016YFB0700804);National Natural Science Foundation of China(NO.T2121004,31830029).

摘　　要：Autologous mosaicplasty is a common approach used to treat osteochondral defects in clinical practice.Gap integration between host and transplanted plugs requires bone tissue reservation and hyaline cartilage regeneration without uneven surface,graft necrosis and sclerosis.However,poor gap integration is a serious concern,which eventually leads to deterioration of joint function.To deal with such complications,this study has developed a strategy to effectively enhance integration of the gap region following mosaicplasty by applying injectable bioactive supramolecular nanofiber-enabled gelatin methacryloyl(GelMA)hydrogel(BSN-GelMA).A rabbit osteochondral defect model demonstrated that BSN-GelMA achieved seamless osteochondral healing in the gap region between plugs of osteochondral defects following mosaicplasty,as early as six weeks.Moreover,the International Cartilage Repair Society score,histology score,glycosaminoglycan content,subchondral bone volume,and collagen II expression were observed to be the highest in the gap region of BSN-GelMA treated group.This improved outcome was due to bio-interactive materials,which acted as tissue fillers to bridge the gap,prevent cartilage degeneration,and promote graft survival and migration of bone marrow mesenchymal stem cells by releasing bioactive supramolecular nanofibers from the GelMA hydrogel.This study provides a powerful and applicable approach to improve gap integration after autologous mosaicplasty.It is also a promising off-the-shelf bioactive material for cell-free in situ tissue regeneration.

关 键 词：MOSAICPLASTY Osteochondral integration Bioactive supramolecular nanofiber GelMA hydrogel Tissue engineering 

分 类 号：TQ427.26[化学工程] R318[医药卫生—生物医学工程]