TGF-β1-supplemented decellularized annulus fibrosus matrix hydrogels promote annulus fibrosus repair  

作　　者：Qiang Wei Dachuan Liu Genglei Chu Qifan Yu Zhao Liu Jiaying Li Qingchen Meng Weishan Wang Fengxuan Han Bin Li 

机构地区：[1]Department of Orthopaedic Surgery,Orthopaedic Institute,The First Affiliated Hospital,Suzhou Medical College,Soochow University,Suzhou,Jiangsu,China [2]Department of Orthopaedic Surgery,The First Affiliated Hospital,Shihezi University School of Medicine,Shihezi,Xinjiang,China [3]Collaborative Innovation Center of Hematology,Soochow University,Suzhou,Jiangsu,China

出　　处：《Bioactive Materials》2023年第1期581-593,共13页生物活性材料（英文）

基　　金：the funding provided for this study by the National Natural Science Foundation of China(81925027,32130059,31872748,32171350,32101103);Natural Science Foundation of Jiangsu Province(BK20200199);China Postdoctoral Science Foundation(2021M702412);the Priority Academic Program Development of Jiangsu Higher Education Institutions.

摘　　要：Annulus fibrosus(AF)repair remains a challenge because of its limited self-healing ability.Endogenous repair strategies combining scaffolds and growth factors show great promise in AF repair.Although the unique and beneficial characteristics of decellularized extracellular matrix(ECM)in tissue repair have been demonstrated,the poor mechanical property of ECM hydrogels largely hinders their applications in tissue regeneration.In the present study,we combined polyethylene glycol diacrylate(PEGDA)and decellularized annulus fibrosus matrix(DAFM)to develop an injectable,photocurable hydrogel for AF repair.We found that the addition of PEGDA markedly improved the mechanical strength of DAFM hydrogels while maintaining their porous structure.Transforming growth factor-β1(TGF-β1)was further incorporated into PEGDA/DAFM hydrogels,and it could be continuously released from the hydrogel.The in vitro experiments showed that TGF-β1 facilitated the migration of AF cells.Furthermore,PEGDA/DAFM/TGF-β1 hydrogels supported the adhesion,proliferation,and increased ECM production of AF cells.In vivo repair performance of the hydrogels was assessed using a rat AF defect model.The results showed that the implantation of PEGDA/DAFM/TGF-β1 hydrogels effectively sealed the AF defect,prevented nucleus pulposus atrophy,retained disc height,and partially restored the biomechanical properties of disc.In addition,the implanted hydrogel was infiltrated by cells resembling AF cells and well integrated with adjacent AF tissue.In summary,findings from this study indicate that TGF-β1-supplemented DAFM hydrogels hold promise for AF repair.

关 键 词：Decellularized matrix Annulus fibrosus HYDROGEL TGF-Β1 Tissue repair 

分 类 号：O63[理学—高分子化学]