电针单穴与腧穴配伍对肠易激综合征模型大鼠结肠功能的影响  

Effect of Electroacupuncture of Single Acupoint and Acupoint Combination on Colonic Function in rats with Irritable Bowel Syndrome Model

作　　者：刘婷婷[1] 陈少宗[1] 晁毓桥 杨凯伟 于燕南 王正文 Liu Tingling;Chen Shaozong;Chao Yuqiao;Yang Kaiwei;Yu Yannan;Wang Zhengwen(Shandong University of Traditional Chinese Medicine,Shandong,Jinan 250355,China)

机构地区：[1]山东中医药大学,山东济南250355

出　　处：《中国中医急症》2023年第4期597-600,605,共5页Journal of Emergency in Traditional Chinese Medicine

基　　金：国家重点研发计划项目(2019YFC1712105);山东省自然科学基金重大基础研究项目(ZR2020ZD17);山东中医药大学科研创新基金(2018-220315)。

摘　　要：目的通过对比观察电针单穴(上巨虚)和腧穴配伍(天枢+上巨虚)对便秘型肠易激综合征(IBS-C)模型大鼠结肠功能调整作用的差异性,初步探讨天枢配伍上巨虚是否有协同作用。方法选用Wistar大鼠90只,分为空白组(10只)和造模组(80只),造模组大鼠采用慢性和急性应激相结合方法连续干预10 d制备IBS-C模型;造模结束后,造模组大鼠通过腹壁撤回反射(AWR)半定量评分结合应激后大便颗粒数与空白组差异有统计学意义,以此筛选制备成功的模型,再随机分为模型组(10只)、上巨虚组(10只)、腧穴配伍组(10只);2个治疗组连续电针干预治疗14 d,对各组大鼠记录结肠痛敏阈值以及结肠肌电;采用酶联免疫吸附法检测各组大鼠血清中乙酰胆碱(ACh)含量;免疫蛋白印迹法检测近端结肠中胆碱乙酰转移酶(ChAT)蛋白表达。结果与空白组相比,模型组痛敏阈值降低(P<0.0001)、结肠慢波频率以及振幅均升高(P<0.001或P<0.01)、血清ACh含量以及近端结肠ChAT蛋白含量均降低(P<0.01);与模型组相比,电针干预治疗后,结肠痛敏阈值升高(P<0.0001)、结肠慢波频率以及振幅降低(P<0.05或P<0.01)、血清Ach含量以及近端结肠ChAT蛋白含量均升高(P<0.01或P<0.05)。结论电针单穴(上巨虚)和腧穴配伍(天枢+上巨虚)均能有效降低IBS-C模型大鼠内脏敏感性和调节结肠动力作用,但天枢配伍上巨虚并未显示明显腧穴配伍的优势和协同作用,针灸干预治疗IBS-C的腧穴配伍组方规律需进一步研究。Objective:To explored whether"Tianshu(ST25)" combined with"Shangjuxu(ST37)"haw a synergistic effect by comparing and observing the dififerences in the effects of electroacupuncture single point(ST37)and acupoint combination(ST25+ST37)on the adjustment of colonic function in IBS-C model rats.Methods:Ninety male clean Wistar rats weighing(200±20)g were selected and divided into a blank group(10 rats)and a modeling group(80 rats),and the IBS-C model was prepared using a combination of chronic and acute stress in the modeling group of rats for 10 consecutive days of intervention;at the end of modeling,the rats in the modeling group were screened by the semi-quantitative score of abdominal wall withdrawal reflex(AWR)combined with the statistically significant number of stool pellets after stress compared with the blank group,and then randomly divided into the model group(10),Shang Jtixti group(10),and Tianshu combined with Shangjuxu group(10);the two treatment groups were treated with continuous electroacupuncture intervention for 14 days,and colonic pain sensitivity thresholds and intestinal electricity were recorded for each group of rats.Detection of ACh in the serum o£each group of rats was conducted by enzyme-linked immunosorbent assay(ELISA);Western blotting was used to detect the expression of proximal colonic ChAT protein expression.Results:Compared with the blank group,the visceral pain threshold in the model group were significantly decreased(P<0.0001),colonic slow wave frequency and amplitude were significantly increased(P<0.001 or P<0.01),serum ACh content and proximal colonic ChAT protein content were decreased(P<0.01).Compared with the model group,the colonic pain sensitivity threshold increased(P<0.0001),colonic slow wave frequency and amplitude were decreased(P<0.05 or P<0.01),serum Ach level and proximal colonic ChAT protein level increased(P<0.01 or P<0.05)after electroacupuncture intervention.Conclusion:Both electroacupuncture of single point(ST37)and acupoint combination(ST25+ST37)are effective i

关 键 词：便秘型肠易激综合征 电针 结肠功能 腧穴配伍 大鼠 

分 类 号：R285.5[医药卫生—中药学]